Nano UHPLC – flow rates of 300nL/min for 75 µm columns
The mass spectrometer has an Orbitrap mass analyzer with a mass range of 50 to 8000 and resolution up to 480,000
Metabolomics
UHPLC – flow rates of 300 uL/min for 2.1 mm diameter columns
The mass spectrometer has an Orbitrap mass analyzer with a mass range of 50-2500 and a resolution of up to 240,000
Studies on the secondary structure, folding and binding properties of proteins
Variable wavelength UV detector. Used for separation of peptides, proteins, and some other molecules, usually with reverse phase columns (1.0-4.6 mm)
Molecular mass measurements for QA/QC of proteins, peptides, small DNA/RNA, drugs, and small molecules by spotting samples with matrix on a plate
Variable detector primarily used for small batch protein purification by SEC but can be fitted for other types of columns (3-10 mm range)
Standard plate reader capable of absorbance, fluorescence, and luminescence
BioLayer Interferometry with disposable probes to measure association and dissociation rates of pairs of molecules and hence dissociation constants. This instrument can perform 8 simultaneous readings. It can also perform quantitation measurements including some equivalent to ELISA.
Concentration or drying of samples in buffers including most organics. Tubes are 0.65-2mL
Drying of samples in mostly aqueous buffer. Uses large glass jars so multiple tubes can be places in each jar.
