Equipment
Type Of Equipment
- Proteomics
- Capillary Electrophoresis (intact mass)
- Nano UHPLC – flow rates of 300nL/min for 75 µm columns
- The mass spectrometer has an Orbitrap mass analyzer with a mass range of 50 to 8000 and resolution up to 480,000
![](https://med.virginia.edu/biomolecular-analysis-facility/wp-content/uploads/sites/330/2023/03/Exploris480-300x295.jpg)
- Metabolomics
- UHPLC – flow rates of 300 uL/min for 2.1 mm diameter columns
- The mass spectrometer has an Orbitrap mass analyzer with a mass range of 50-2500 and a resolution of up to 240,000
![](https://med.virginia.edu/biomolecular-analysis-facility/wp-content/uploads/sites/330/2023/03/ID-X-263x300.jpg)
- Studies on the secondary structure, folding and binding properties of proteins
![](https://med.virginia.edu/biomolecular-analysis-facility/wp-content/uploads/sites/330/2023/03/Jasco_J1500-275x300.jpg)
- Variable wavelength UV detector. Used for separation of peptides, proteins, and some other molecules, usually with reverse phase columns (1.0-4.6 mm)
![](https://med.virginia.edu/biomolecular-analysis-facility/wp-content/uploads/sites/330/2023/03/Ultimate3000_HPLC-160x300.jpg)
- Molecular mass measurements for QA/QC of proteins, peptides, small DNA/RNA, drugs, and small molecules by spotting samples with matrix on a plate
![](https://med.virginia.edu/biomolecular-analysis-facility/wp-content/uploads/sites/330/2023/03/Shimadzu8030-166x300.jpg)
- Variable detector primarily used for small batch protein purification by SEC but can be fitted for other types of columns (3-10 mm range)
![](https://med.virginia.edu/biomolecular-analysis-facility/wp-content/uploads/sites/330/2023/03/AKTAPure-237x300.jpg)
- Standard plate reader capable of absorbance, fluorescence, and luminescence
![](https://med.virginia.edu/biomolecular-analysis-facility/wp-content/uploads/sites/330/2022/03/platereader-300x283.jpg)
- Concentration or drying of samples in buffers including most organics. Tubes are 0.65-2mL
![](https://med.virginia.edu/biomolecular-analysis-facility/wp-content/uploads/sites/330/2024/02/SpeedVac.jpg)
- Drying of samples in mostly aqueous buffer. Uses large glass jars so multiple tubes can be places in each jar.
![](https://med.virginia.edu/biomolecular-analysis-facility/wp-content/uploads/sites/330/2024/02/Lyophilizer.jpg)