Equipment

The Biomolecular Analysis Facility has equipment which is either not commonly found in individual laboratories or is needed only occasionally by some laboratories. Some equipment is operated by facility personnel, and some is used by individual researchers.

For more information: contact Nicholas Sherman ph. 924-0070

Statement of resources for grant applications

TYPE OF EQUIPMENT IMAGE
Thermo Scientific Q Exactive HF-X
We have four methods of loading samples into this instrument:

  • infusion pump (no fractionation)
  • Capillary electrophoresis
  • nano UHPLC-flow rates of nnn for 75 µm diamater columns
  • UHPLCs with flow rates up to 1 ml/min for larger columns

The mass spectrometer has an Orbitrap mass analyzer with a mass range of 50 to 8000, and resolution up to 240,000.

q-exactive machine
Bruker MicroFlex MALDI
This instrument measures masses of intact molecules. Sample preparation is usually relatively simple so this instrument is the first choice to obtain the mass of peptides, proteins and some other molecules.
bruker MALDI time of flight mass spectrometer
Molecular Dynamics Gemini EM SpectraMax Fluorescent plate reader

  • For fluorescence readings of plates with 384, 96, 24, 12, 6 well microtiter plates.
  • Dual monochromator optics.
  • Does not perform absorbance readings.
  • Operated by researcher. Fee for use.
fluorescence plate reader
High performance liquid chromatography.

  • Dionex Ultimate for 0.3 to 4.6 mm diameter columns.

Variable wavelength UV detector.Used for separation of peptides, proteins and some other molecules, usually with reverse phase columns.

Dionex HPLC
JASCO J-1500 Circular dichroism spectropolarimeter
For studies on tertiary structure of proteins.
Operated by researcher. Fee for use.
Brief background on operation here.
Molecular interactions
fortéBIO Octet RED96 uses BioLayer Interferometry with disposable probes to measure association and dissociation rates of pairs of molecules and hence dissociation constants.
This instrument can perform 8 simultaneous readings. It can also perform quantitation measurements including some equivalent to ELISA,
fortéBIO Octet
Acea RTCA cell analysis instrument
real time, continuous measurement of cellular activity, including migration, based on impedance measurements
ACEA RTCA instrument
Biotek ELx800 absorbance plate reader
absorbance measurements on microtiter plates at 450, 562, 600, 660 nm
BioTek Absorbance reader