Equipment

The Biomolecular Analysis Facility has equipment which is either not commonly found in individual laboratories or is needed only occasionally by some laboratories. Some equipment is operated by facility personnel, and some is used by individual researchers.

For more information: contact Nicholas Sherman ph. 924-0070

Statement of resources for grant applications

 

TYPE OF EQUIPMENT IMAGE

Thermo Scientific Q Exactive HF-X

We have four methods of loading samples into this instrument:

  • infusion pump (no fractionation)
  • Capillary electrophoresis
  • nano UHPLC-flow rates of nnn for 75 µm diamater columns
  • UHPLCs with flow rates up to 1 ml/min for larger columns

The mass spectrometer has an Orbitrap mass analyzer with a mass range of 50 to 8000, and resolution up to 240,000.

 

Q-Exactive mass spectrometer

Bruker MicroFlex MALDI

This instrument measures masses of intact molecules. Sample preparation is usually relatively simple so this instrument is the first choice to obtain the mass of peptides, proteins and some other molecules.

bruker MALDI time of flight mass spectrometer

Molecular Dynamics iD3 multi-mode plate reader

  • For fluorescence readings of plates with 384, 96, 24, 12, 6 well microtiter plates.
  • Luminescence readings
  • reagent injectors
  • Dual monochromator optics.
  • absorbance readings.
  • Operated by researcher. Fee for use.
iD3 multi-mode plate reader

High performance liquid chromatography.

  • Dionex Ultimate for 0.3 to 4.6 mm diameter columns.

Variable wavelength UV detector.Used for separation of peptides, proteins and some other molecules, usually with reverse phase columns.

Dionex HPLC

JASCO J-1500 Circular dichroism spectropolarimeter

For studies on tertiary structure of proteins.
Operated by researcher. Fee for use.
Brief background on operation here.

JASCO J-1500 CD instrument

fortéBIO Octet RED96 uses BioLayer Interferometry with disposable probes to measure association and dissociation rates of pairs of molecules and hence dissociation constants.
This instrument can perform 8 simultaneous readings. It can also perform quantitation measurements including some equivalent to ELISA,

fortéBIO Octet

Acea RTCA cell analysis instrument

real time, continuous measurement of cellular activity, including migration, based on impedance measurements

ACEA RTCA instrument