UVA School of Medicine

Speaker: Dr. Peter Yingxiao Wang

Professor of Bioengineering
Department of Bioengineering
Institute of Engineering in Medicine
University of California, San Diego

Host: Dr. Huiwang Ai

Speaker: Dr. Mustafa Mir

Postdoctoral Researcher
Department of Molecular and Cell Biology
University of California, Berkeley

Host: Dr. Anne Kenworthy

Speaker: Dr. Irina Kaverina

Professor of Cell and Developmental Biology
Vanderbilt University

Host: Dr. Anne Kenworthy

Speaker: Dr. Meghan Morrissey

Postdoctoral Fellow
Department of Cellular and Molecular Pharmacology, University of California, San Francisco

Host: Dr. Anne Kenworthy

Speaker: Dr. Ilya Levental

Associate Professor of Integrative Biology and Pharmacology
McGovern Medical School at the University of Texas Health Science Center at Houston

Host: Dr. Anne Kenworthy

Speaker: Dr. Or Shemesh

Postdoctoral Fellow
Synthetic Neurobiology, MIT Media Lab and McGovern Institute for Brain Research
Massachusetts Institute of Technology

Host: Dr. Anne Kenworthy

Speaker: Dr. Erik Welf

Instructor
Departments of Cell Biology and Bioinformatics

UT Southwestern Medical Center

Host: Dr. Anne Kenworthy

Speaker: Dr. Ruobo Zhou

HHMI Postoctoral Fellow of the Life Sciences Research Foundation
Department of Chemistry and Chemical Biology
Harvard University

Host: Dr. Anne Kenworthy

Speaker: Dr. Takjip Ha, Bloomberg Professor of Biophysics and Biomedical Engineering, Johns Hopkins University

Host: Dr. Lukas Tamm, Chair, Molecular Physiology and Biological Physics, and Director of the Center for Membrane & Cell Physiology.

Speaker: Dr. Robert Campbell

Professor of Science, Chemistry
University of Alberta, Canada and University of Tokyo, Japan

Host: Dr. Huiwang Ai

Abstract: The Campbell research group is focused on the use of protein engineering for the development of fluorescent protein-based biosensors for imaging of cell signalling and metabolism. Protein engineering, using a combination of rational protein design and directed protein evolution, is the most effective and versatile approach for generating new genetically encoded fluorescent biosensors. By exploiting structure-guided design, iterative cycles of high-throughput fluorescence image-based screening of bacterial colonies, and lower throughput testing of promising variants in mammalian cells, we are developing a growing selection of fluorescent protein-based biosensors with improved properties. In this seminar I will present some of our most recent efforts to engineer an improved generation of biosensors. Specifically, I will provide an update on the expanding palette of calcium ion biosensors, and describe how we are using similar engineering efforts to make biosensors for neurotransmitters, ions, and key metabolites.