The Flow Cytometry Core Facility, housed in Pinn Hall (Room 2011 and 2013), provides all investigators at the University of Virginia access to high quality, cost effective flow cytometry services. By providing these services, as well as the scientific expertise necessary to effectively use this technology, the facility serves to enhance the scope and quality of scientific research performed at the University.

With state of the art instrumentation, such as the CyTOF 2 mass cytometer with Helios upgrade and the 5 laser 15 color BD Influx Cell Sorter in a Class II BSC, the facility offers up to 6-way high-speed cell sorting up to BSL2+ and complex analytical services. The facility has two additional cell sorters; a 5 laser 15 color FACSAria Fusion (also in a customized Class II BSC), and a FACSVantage SE TurboSort DIVA equipped with four lasers: 488nm, 405nm, 642nm and 592nm (642nm and 592nm collinear), both capable of 4-way high-speed cell sorting. The facility also has a variety of other bench top analytical instruments which include a 3 laser 5-color and 2 laser 4-color FACSCalibur bench top analyzers; a Cytek upgraded 4-laser 10-color FACSCalibur with 96 well autosampler; a 9-color CyAn ADP LX benchtop cytometer, an LSRFortessa with 17 fluorescent detectors, a 4 laser 14 color Attune NxT with 96 well autosampler, and two recently added Aurora Spectral cytometers capable of >20 colors. Additional capabilities include an ImageStreamX Mark II imaging flow cytometer and two Luminex MAGPIX bead-based multiplex analyzers, with complete multiplex assay services for a variety of human and mouse cytokine/chemokine and signaling panels.

The Flow Cytometry Core offers full week training courses quarterly that include didactic as well as hands on training. Researchers have the option, once trained, of performing their own acquisition/analysis or utilizing the expertise of the facility’s staff to run their samples for them.

This Core is supported through the University of Virginia Cancer Center National Cancer Institute P30-CA044579-23 Center Grant (please reference this grant number in all publications).

Acknowledgement of the core facility in all publications which utilized the service of the core is customary and appreciated, as it is used as a value based metric in determining future funding.

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FCS Express Workshops (Pinn Hall Teaching Labs 2006, 10am-11am)