The Flow Cytometry Core Facility is housed in Pinn Hall (Room 2011 and 2013) and provides all investigators at the University of Virginia access to high quality, cost effective flow cytometry services. By providing these services, as well as the scientific expertise necessary to effectively use this technology, the facility serves to enhance the scope and quality of scientific research performed at the University.

With state of the art instrumentation, such as the CyTOF 2 mass cytometer with Helios upgrade and the 5 laser 15 color BD Influx Cell Sorter in a Class II BSC, the facility offers up to 6-way high-speed cell sorting up to BSL2+ and complex analytical services. In addition,the facility has two additional cell sorters; a FACSVantage SE TurboSort DIVA capable for 4-way high-speed cell sorting and a 5 laser 15 color FACSAria Fusion (also in a customized ClassII BSC). The facility also has a variety of other bench top analytical instruments which include two 2-laser 4-color FACSCalibur bench top analyzers; a Cytek upgraded 4-laser 10-color FACSCalibur with 96 well autosampler; a 9-color CyAn ADP LX benchtop cytometer, an LSRFortessa with 17 fluorescent detectors, and a recently added 4 laser 14 color Attune NxT with 96 well autosampler. Additional capabiites include an ImageStreamX Mark II imaging flow cytometer and two Luminex MAGPIX bead-based multiplex analyzers, with complete multiplex assay services for a variety of human and mouse cytokine/chemokine panels .

The Flow Cytometry Core regularly offers full week training courses that include didactic as well as hands on training.

Researchers have the option, once trained, of performing their own acquisition/analysis or utilizing the expertise of the facility’s staff to run their samples for them.

This Core is supported through the University of Virginia Cancer Center National Cancer Institute P30-CA044579-23 Center Grant (please reference this grant number in all publications).

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FCS Express Workshops (Pinn Hall Teaching Labs 2006, 10am-11am)

May 15th
Gating:
We will cover creating and modifying gates and their hierarchy on your data to enable you to better drill down to populations of interest. This workshop will cover topics such as data-specific gates, or gates that change between your samples; how to use markers on 1-dimensional plots such as histograms; back-gating strategies to identify subpopulations; setting up quadrant gating; and how to derive statistical information from the gates you have applied to your data. This workshop will cover information useful to new flow analysts and more experienced users alike. Information learned here will be helpful in later workshops, specifically in regards to compensation and spreadsheets.
June 12th
Compensation:
In this workshop we will cover how to generate a compensation matrix for multi-color flow experiments using 4-color demonstration data. For experiments that use mixed media compensation ie beads and cells, we will go over how to set up gates on your plots so the correct population is displayed. We will also talk about switching between multiple compensation matrices, using a compensation matrix acquired on a cytometer, and tips and tricks on how to compensate samples that exhibit proteins in fluorescent transition states, such as pH sensitive dyes. This workshop will cover information useful to all users who intend to analyze multi-parameter flow cytometry and will make use of some techniques taught in the Gating workshop.