CyTOF XT – Resources
Webinars
- Decoding Tumors with Mass Cytometry by Jonathan Irish, PhD, a discussion of systems-level characterization of cells in tumor.
Protocols
- Mass Cytometry Methods and Protocols
- Immunophenotyping of Human Peripheral Blood Mononuclear Cells by Mass Cytometry (Heck S, Bishop CJ, Ellis RJ
Methods Mol Biol. 2019, 1979, 285-303)
- Preparing Viable Single Cells from Human Tissue and Tumors for Cytomic Analysis (Leelatian N, Doxie DB, Greenplate AR, Sinnaeve J, Ihrie RA, Irish JM. Curr Protoc Mol Biol. 2017)
- Phenotyping of Live Human PBMC using CyTOF Mass Cytometry (Leipold and Maecker 2015)
- Intracellular Cytokine Staining on PBMCs Using CyTOF Mass Cytometry (Lin, Gupta, and Maecker 2015)
- Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry
- A Deep Profiler’s Guide to Cytometry
- Mass Cytometry Selected Publications
- Standard BioTools – Metal-Conjugated Antibodies
- Panel Design Guidelines
- Experiment Planning Worksheet
- Cell Surface Staining Protocol
- Cytoplasmic Staining Protocol
- Intercalator Ir-125 Protocol
- Intercalator Rh500 uM (Live Dead) Protocol
- Phospho-Protein Staining Protocol
- Nolan Lab Signaling based Resource (Fluorescence & Mass)
- BD Buffer/Fixative Resource