About

Our Goals
Resource Statement

We encourage users to acknowledge the core in their posters and publications. A resource statement summarizes the capabilities of the core.

See Statement

The mission of the Genome Analysis and Technology Core (GATC)  is to serve as a scientific resource and biotechnology hub enhancing the scope and quality of basic and translational research at UVA, and to provide customer satisfaction, collaboration and quality control.

The goal of the core staff is to enable Next Generation Sequencing projects for both novice and experienced users alike, by providing timely assistance in all facets of experimentation from project design and execution to data analysis and reporting.

Core Acknowledgement

NEW: All publications using data generated by the GATC core must mention the name of the core and its RRID# in the acknowledgement / or methods section.  Contact us if you need help with your method section.

Example: RNA samples were processed for library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit (Illumina), according to validated standard operating procedures established by the UVA School of Medicine’s Genome Analysis and Technology Core (RRID:SCR_018883).

Publications resulting from research supported by a Cancer Center co-pay  must mention the core by name in materials and methods (as above in bold) and include the following in the acknowledgment:  “This research was supported by the NCI Cancer Center Support Grant 5P30CA044579“.

For scientific contribution to the published data by members of the core, consider adding their names to the co-Author list.

Meet the Team

Katia Sol-Church, PhD., is the current Director overseeing the activities of the GATC.  Dr. Sol-Church is a Professor in the Department of Pathology, with 19 years of experience in core operation.  She provides mentoring and consultations for project design and troubleshooting, coordination for sample processing and for the development of new services supporting Next Generation Sequencing.  Katia  has a long-standing record of successful collaborative research with clinicians and scientists, including early career scientists, leading to the publication of 68 peer reviewed articles and one Book Chapter on RASopathies.   Dr. Sol-Church is a recognized leader in the core community for her work in support of education, research rigor and reproducibility.

Yongde Bao, Ph.D., is Associate Professor of Research with expertise in analytical chemistry and Biotechnology including bioinformatics analysis of gene expression and NGS data set.  Yongde  is a veteran in the lab with 24 years of experience in core operation.  He was instrumental in establishing the first core NGS services at UVa.  Dr. Bao currently lead the daily operation of the GATC sequencing facility located in room 1044 and provides data analysis services and guidance for project design.  Dr. Bao has been active with the core community as a research group member, notably with the Association of Biomolecular Resource Facilities.   He is also ad hoc editor/reviewer for a number of biotechnical journals.

 Alyson Prorock, M.S., leads the daily operation of the GATC preparative lab located in room 1076, and functions as back up to the sequencing core.   Alyson brings to the core a unique set of skills due to her undergraduate training in process engineering and her graduate training in biomedical engineering.  Though her primary responsibility is to execute new workflow for NGS library preparation, she is instrumental at maintaining rigor and reproducibility in all aspects of core services.  Alyson provides guidance and troubleshooting to users and facilitate iLab access to the core services.

Services and Location

Located in Pinn Hall, the core is a fee-for-service facility that offers instrumentation and expertise in all areas of NGS genomics and transcriptomics.   Current applications supported by the core staff include 10x Genomics library preps (including Single Cell (Sc) RNA Seq, ScATAC-seq, CNV, VDJ Immune profiling, Sc proteogenomics  and Cite-seq with Feature barcoding), bulk RNA Seq, CHIP Seq, ATAC Seq, Amplicon DNA Seq, 16S ribosomal gene sequencing, shot gun sequencing for small genomes and whole exome sequencing (WES).  The core also provides real time and droplet digital PCR services most suitable for targeted gene expression, SNP genotyping and CNV discovery.  GATC provides training for the core shared instrumentation used for nucleic acid analysis.

Two technologies under development include Nanopore long read  sequencing, and VISIUM spatial transcriptomics.

 

More Information

The GATC team also provides multi-core project coordination to streamline project and enhance data output.  We provide student training and education, and support for  grant application including project design, letter of support and budget preparation.