Rates-table
Next Gen Sequencing
| Service | University of Virginia and affiliates | University of Virginia Cancer Center Members | Academic external |
|---|---|---|---|
| NGS Run Setup (add to MiSeq and Next Seq cycle sequencing orders) | $231.00 | $173.25 | $371.91 |
| MiSeq kit, 50 cycles | $810.00 | $607.50 | $850.00 |
| MiSeq kit, 150 cycles (1 x 150 bp, 2 x 75 bp) | $885.00 | $663.75 | $929.25 |
| MiSeq kit, 300 cycles (1 x 300 bp, 2 x 150 bp) | $1025.00 | $768.75 | $1076.25 |
| MiSeq kit, 500 cycles (1 x 500 bp, 2 x 250 bp) | $1,150.00 | $862.50 | $1,207.50 |
| MiSeq kit, 600 cycles (1 x 600 bp, 2 x 300 bp) | $1485.00 | $1113.75 | $1559.25 |
| NextSeq kit – 75 cycle High Output Kit | $1,410.00 | $1057.5 | $1,480.50 |
| NextSeq kit – 150 cycle High Output Kit | $2,675.00 | $2,006.25 | $2,808.75 |
| NextSeq kit – 150 cycle Mid Output Kit | $1,040.00 | $780.00 | $1,092.00 |
| NextSeq kit – 300 cycle High Output Kit | $4,260.00 | $3,195 | $4,473.00 |
| NextSeq kit – 300 cycle Mid Output Kit | $1,650.00 | $1,237.50 | $1,732.50 |
| Next Gen Sequencing data analysis, per hour | $85.00 | $63.75 | $136.85 |
NGS Library Preparation Services
| Service | University of Virginia and affiliates | University of Virginia Cancer Center Members | Academic external |
|---|---|---|---|
| NGS library prep 10X Genomics-single cell RNAseq | $1,750.00 | $1,312.50 | $2,817.50 |
| NGS library prep-Nextera XT | $70.00 | $52.50 | $112.70 |
| NGS library prep RNA-seq M using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® | $262.00 | $196.50 | $421.82 |
| NGS library prep RNA-seq R using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® and NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) | $360.00 | $270.00 | $579.60 |
| NEB small RNAseq | $85.00 | $63.75 | $136.85 |
| NGS library prep-16s bacteria | $50.00 | $37.50 | $80.50 |
| Pippin Prep, core run per sample (4 or more samples) | $22.00 | $16.50 | $35.42 |
| Pippin Prep, user run per cartridge (user provided consumables) | $30 | $22.50 | $48.30 |
| Covaris use for DNA shearing – 1st sample, user operated | $45.00 | $33.75 | $72.45 |
| Covaris use, each additional sample, user operated |
$20.00 | $15.00 | $32.20 |
| SPRIselct bead cleanup | $25 | $18.75 | $40.25 |
Quality control and quantitation services
| Service | University of Virginia and affiliates | University of Virginia Cancer Center Members | Academic external |
|---|---|---|---|
| Quantitation by absorbance with NanoView | $2.00 | $1.50 | $3.22 |
| Quantitation by fluorescence with QuBit | $3.00 | $2.25 | $4.83 |
| Agilent Tape Station using RNA kit | $12.00 | $9 | $19.32 |
| Agilent Tape Station using RNA HS kit | $13.00 | $9.75 | $20.93 |
| Agilent Tape Station user run | $30.00 | $22.50 | $48.30 |
| Agilent Tape Station training for user run | $30.00 | $22.50 | $48.30 |
| Agilent Bioanalyzer using DNA HS kit | $13.00 | $9.75 | $20.93 |
| Agilent Bioanalyzer, user run | $25.00 | $18.75 | $40.25 |
PCR Services
| Service | University of Virginia and affiliates | University of Virginia Cancer Center Members | Academic external |
|---|---|---|---|
| qPCR-QuantStudio 6 Flex plate run |
$85 | $63.75 | $136.85 |
| qPCR-QC for NGS library |
$12 | $9.00 | $19.32 |
| ddPCR -user operated – first 8 samples | $45 | $33.75 | $72.45 |
| ddPCR – user operated – additional 8 samples | $25 | $18.75 | $40.25 |
| ddPCR-analysis by core-first 8 samples |
$65 | $48.75 | $104.65 |
| ddPCR analysis by core – additional 8 samples | $45 | $33.75 | $72.45 |