Methods and Resources
Methods and Resources
The UVa Molecular Electron Microscopy Core laboratory is a state-of-the-art facility for high-resolution cryo-EM and cryo-ET. It houses four microscopes: a 300 kV Titan Krios, 200 kV Glacios, 200 kV F20, and 120 kV Spirit. MEMC staff provide support ranging from sample preparation to full image collection. Services include staining, embedding, sectioning with an EM UC7 ultramicrotome, plunge freezing, automated loading for Krios and Glacios, and cryo-FIB milling with the Aquilos 2 for cryo-ET lamella preparation. Training is available for most procedures, including microscope operation.
Core Director: Michael Purdy
The biomolecular nuclear magnetic resonance facility at UVa is home to one 800, three 600, and two 500 MHz spectrometers, which are equipped with triple-resonance cryoprobes. A 500 MHz solid-state NMR spectrometer with a heteronuclear MAS-probe is available for solids experiments.
More: https://med.virginia.edu/biomolecular-magnetic-resonance-facility/
Core Director: Jeff Ellena
EPR equipment includes two X-band cw spectrometers, a pulse X-band machine capable for DEER, and a pulse Q band spectrometer.
Robotics for membrane protein crystallization is available. These instruments can set up batch or vapor diffusion experiments and the protein crystals can be visualized and docmented using polarized visible light or UV fluorescence microscopes at 4 and 17 degrees Celsius.
Research Faculty: Michael Purdy
The Center houses culture facilities for large-scale expression in bacterial, yeast, insect, and mammalian cell systems. Protein purifications and characterizations are handled by individual labs and collaboratively as needed.
Research Faculty: Mark Daniels
Confocal, TIRF, lifetime, and laser microscopes for cell biological research and single molecule detection are available to Center researchers.
Research Faculty: Volker Kiessling, Kandice Levental
For University and School of Medicine-wide Cores, see:
W. M. Keck Center for Cellular Imaging
Director: Ammasi Periassamy
Director: Sijie Hao
Synchrotron beam time is available at the Argonne National Lab Advanced Photon Source.
Research Faculty: Michael Purdy
Contact: Huiwang Ai
Contact: Ilya Levental & Huan Bao