Current Information
The PCEN has been successful in attracting and retaining talented undergraduate and graduate students, postdocs, and junior faculty to research in Pediatric Nephrology.
Hoang Yen Le, rising 4th year undergrad student, University of Virginia. Mentors: R. Ariel Gomez MD and Maria Luisa S. Sequeira-Lopez MD, UVA Pediatrics.
During her 2024 summer intership, Hoang learned how to performed tissue clearing and 3D imaging of mouse kidneys, renal denervation surgery and immunostaining. She presented her work at the KUH Summer Undergraduate Research Symposium, July 31-August 2, 2024. University of Virginia.
- Ahana Sinharoy*, Hoang Yen Ngoc Le*, Manako Yamaguchi MD PhD, Lucas Ferreira de Almeida MS PhD, Hiroki Yamaguchi MD PhD, Silvia Medrano PhD, Maria Luisa S. Sequeira Lopez MD PhD, R. Ariel Gomez MD PhD * Equal contribution. Three-Dimensional Visualization of Concentric Renal Arteriolar Hypertrophy in Renin Gene Knockout Mouse. KUH Summer Undergraduate Research Symposium, July 31-August 2, 2024. University of Virginia.
Hoang will continue to work in the Gomez/Sequeira-Lopez labs as a student volunteer during the 2024-2025 school year.
Arianna Romero-Gonzalez, rising 2rd year undergrad student, Piedmond Virginia Community College. Mentors: R. Ariel Gomez MD and Maria Luisa S. Sequeira-Lopez MD, UVA Pediatrics
During her 2024 summer internship, Ari trained in histology and immunohistochemical techniques, microscopy, mouse genetics and physiology. She presented her work at the KUH Summer Undergraduate Research Symposium, July 31-August 2, 2024. University of Virginia.
- Gigi Toirova*, Arianna Romero-Gonzalez*, Lucas Ferreira de Almeida MS PhD, Silvia Medrano PhD, Xuiyin Liang, Maria Luisa S. Sequeira-Lopez MD, R. Ariel Gomez MD * Equal contribution. Reversibility of Acute Renin Cell Recruitment in the Mouse. KUH Summer Undergraduate Research Symposium, July 31-August 2, 2024. University of Virginia.
Ari will continue to work in the Gomez/Sequeira-Lopez lab as a student volunteer during the 2024-2025 school year.
Gigi Toirova, rising 4rd year undergrad student, University of Virginia, Mentors: R. Ariel Gomez MD and Maria Luisa S. Sequeira-Lopez MD, UVA Pediatrics.
During her 2024 summer intership, Gigi trained in histology and immunohistochemistry techniques, microscopy, mouse genetics and physiology. She presented her work at the KUH Summer Undergraduate Research Symposium, July 31-August 2, 2024. University of Virginia.
- Gigi Toirova*, Arianna Romero-Gonzalez*, Lucas Ferreira de Almeida MS PhD, Silvia Medrano PhD, Xuiyin Liang, Maria Luisa S. Sequeira-Lopez MD, R. Ariel Gomez MD * Equal contribution. Reversibility of Acute Renin Cell Recruitment in the Mouse. KUH Summer Undergraduate Research Symposium, July 31-August 2, 2024. University of Virginia.
Mohamed Diagne, BS, University of Virginia. Mentors: R. Ariel Gomez MD and Maria Luisa S. Sequeira-Lopez MD, UVA Pediatrics, and Nick Guagliardo PhD, UVA Pharmacology.
During his 2023 summer intership, Mohamed trained in histology and immunohistochemistry techniques, microscopy and mouse genetics in the Gomez/Sequeira-Lopez lab. He gave a talk at the PCEN-Lunchtime Learning Sessions: Current Research on Primary Hyperaldosteronism. July 19th, 2023.
During his 2024 PCEN summer intership, Mohamed conducted Ca2+ imaging studies in the adrenal gland of mammals under the mentorship of Dr. Nick Guagliardo. He later joined the Harvard 2024 RSI program to train in Dr. Fernando Camargo’s Lab at Boston Children’s Hospital.
Iden Han, summer student at Children’s National Hospital, DC. Mentor: Patricio Ray MD, UVA Pediatrics.
Jesus Neyra, BS Biology and Environmental Sciences, University of Virginia. Mentors: Maria Luisa S. Sequeira-Lopez MD, Silvia Medrano PhD, and R. Ariel Gomez MD, UVA Pediatrics.
Jesus joined the Gomez/ Sequeira-Lopez labs in the spring of 2022 as a 3rd year undergrad student and stayed through May 31st, 2024. He did two summer internships in the lab, sponsored in part by a Biochemical Society Studentship (London, UK) in 2022 and the UVA School of Medicine SRIP in 2023, to work on the role of transcription factors in renin cell identity. Currently, he is a student in the Medical Scientist (MD/PhD) Training Program at the University of Maryland, Baltimore. Jesus has presented his work at national and international meetings, and published an original article as first author in the American J Physiology Renal.
Publications
Neyra JS, Medrano S, Goes Martini A, Sequeira-Lopez MLS, Gomez RA. The role of Gata3 in renin cell identity. Am J Physiol Renal Physiol. 2023 Aug 1;325(2):F188-F198. doi: 10.1152/ajprenal.00098.2023. Epub 2023 Jun 22. PMID: 37345845; PMCID: PMC10396225
Presentations
Neyra JS, Silvia Medrano, Maria Luisa S. Sequeira-Lopez, R. Ariel Gomez. Role of GATA3 in Renin Cell Identity. CHRC 34th Annual Research Symposium, UVA. Poster presentation. May, 2022
Neyra JS, Silvia Medrano, Maria Luisa S. Sequeira-Lopez, R. Ariel Gomez. Role of GATA3 in Renin Cell Identity. APS/ASN Control of Renal Function in Health and Disease Conference, Charlottesville, VA. Oral presentation. June, 2022.
Neyra JS, Medrano S, Sequeira-Lopez MLS, and Gomez RA. The Role of Gata3 in Renin Cell Identity. AHA Council on Hypertension Meeting. San Diego, CA. Poster presentation. September 2022. https://doi.org/10.1161/hyp.79.suppl_1.P091.
Neyra JS, Medrano S, Sequeira-Lopez MLS, and Gomez RA. The Role of Gata3 in Renin Cell Identity. Annual Child Heath Research Trainee Competition, UVA. Oral presentation. 1st Place Winner. May 2023.
Neyra JS, Medrano S, Sequeira-Lopez MLS, and Gomez RA. The Role of Gata3 in Renin Cell Identity. 35th Annual Child Heath Research Symposium, UVA. Oral presentation. May 2023.
Neyra JS, Medrano S, Goes Martini A, Sequeira-Lopez MLS, and Gomez RA. The Role of Gata3 in Renin Cell Identity. Annual UVA School of Medicine Summer Research Internship Program Symposium. Oral presentation. August 2023.
Neyra JS, Medrano S, Alexandre G Martini, Sequeira-Lopez MLS, and Gomez RA. The Role of Gata3 in Renin Cell Identity. AHA Council on Hypertension Meeting, Boston, MA. Poster presentation. September 2023. https://doi.org/10.1161/hyp.80.suppl_1.P224.
Neyra JS, Medrano S, Goes Martini A, Sequeira-Lopez MLS, and Gomez RA. The Role of Gata3 in Renin Cell Identity. American Society of Nephrology (ASN)/APS: Kidney Week & Early Career Kidney Conference. Poster presentation. November 2023.
Neyra JS*, Medrano S, Goes Martini A, Sequeira-Lopez MLS, and Gomez RA. Gata 3 and localization of renin cells in the kidney. Pediatric Center of Excellence in Nephrology (PCEN) Network Symposium. Oral presentation. February 2024.
Neyra JS, Medrano S, Sequeira-Lopez MLS, Gomez RA. GATA3: A Regulator of Renin Cell Development and Blood Pressure. 36th Annual Child Health Research Symposium, UVA. Poster presentation. 1st Place Winner. May 2024.
Ahana Sinharoy, 4th year undergrad, University of Virginia. Mentors: R. Ariel Gomez MD and Maria Luisa S. Sequeira-Lopez MD, UVA Pediatrics.
Ahana has been training in the Gomez/ Sequeira-Lopez labs as a volunteer since August 2023. She is working on renin cells in the placenta and vascular innervation in the kidney. She presented her work at the KUH Summer Undergraduate Research Symposium, July 31-August 2, 2024. University of Virginia
Abstracts and Presentations:
Yamaguchi M, Sinharoy A, Yamaguchi H, Almeida LF, Liang X, Smith JP, Medrano S, Sequeira-Lopez MLS, Gomez RA. Hyperinnervation of renin cells and renal arterial tree during long – term inhibition of the renin-angiotensin system (RAS). 36th Annual Child Health Research Trainee Competition and Symposium, UVA. Oral and poster presentation. 4th Place Winner (MY). May 2024.
Ahana Sinharoy*, Hoang Yen Ngoc Le*, Manako Yamaguchi MD PhD, Lucas Ferreira de Almeida MS PhD, Hiroki Yamaguchi MD PhD, Silvia Medrano PhD, Maria Luisa S. Sequeira Lopez MD PhD, R. Ariel Gomez MD PhD * Equal contribution. Three-Dimensional Visualization of Concentric Renal Arteriolar Hypertrophy in Renin Gene Knockout Mouse. KUH Summer Undergraduate Research Symposium, July 31-August 2, 2024. University of Virginia.
Rishi Raghavan, BS, University of Virginia. Mentors: R. Ariel Gomez MD and Maria Luisa S. Sequeira-Lopez MD, UVA Pediatrics, and Nick Guagliardo PhD, UVA Pharmacology.
Rishi joined the Gomez/Sequeira-Lopez labs as a rising UVA 4th year student for a summer intership in 2023. He trained in histology and immunohistochemistry techniques, microscopy and mouse genetics. He continue in the Gomez/Sequeira-Lopez labs as a student volunteer during the 2023-2024 school year to work on Ca2+ imaging in juxtaglomerular cells of the murine kidney. He is currently an MD student at Virginia Commonwealth University.
Meghan Luellen, Department of Biomedical Engineering, UVA: Capstone Biomedical Research Program 2023-24. Standardization and optimization of urinary extracellular vesicle isolation by modifying hydration of healthy cohort. Mentor: Agnieszka Swiatecka-Urban, UVA Pediatrics.
Grace Boitnott, VA K Tutor Program, summer 2024. Standardization and optimization of urinary extracellular vesicle isolation by modifying hydration of healthy cohort. Mentor: Agnieszka Swiatecka-Urban, UVA Pediatrics.
Graduate Students:
Bingjie Xue, PhD student in Biomedical Engineering, UVA. Mentors: R. Ariel Gomez MD, UVA Pediatrics and Nathan Sheffield, PhD, UVAVCenter for Public Health Genomics.
Bing’s work focusses on Integrative Computational Aanalysis of multi-omics data to define renin cell identity.
Publications
Xue B, Khoroshevskyi O, Gomez RA, Sheffield NC. Opportunities and challenges in sharing and reusing genomic interval data. Front Genet. 2023 Mar 20;14:1155809. doi: 10.3389/fgene.2023.1155809. PMID: 37020996; PMCID: PMC10067617.
Presentations
Xue B, Medrano S, Smith JP, Martini AG, Sheffield NC, Sequeira-Lopez MLS and Gomez RA. Specific Chromatin Configurations Across the Genome Determine the Renin Cell Identity. AHA Council on Hypertension Meeting. Poster presentation. September 2022, https://doi.org/10.1161/hyp.79.suppl_1.P116 Hypertension 2022.
Xue B, Medrano S, Smith JP, Martini AG, Sheffield NC, Sequeira-Lopez MLS, and Gomez RA. The Reninness Score: Integrative Analysis of Multi-Omic Data to Define Renin Cell Identity. 35th Annual Child Heath Research Symposium, UVA. Poster presentation. May 2023.
Xue B, Medrano S, Smith J, Martini A, Sheffield N, Sequeira-Lopez MLS, Gomez RA.The reninness score: integrative analysis of multi-omic data to define renin cell identity. AHA Council on Hypertension Meeting, Boston, MA. Poster presentation. https://doi.org/10.1161/hyp.80.suppl_1.P225. Winner of the Trainee Advocacy Committee of the Council on Hypertension Travel Award. September 2023.
Xue B, Medrano S, Smith JP, Martini A, Sheffield NC, Sequeira-Lopez MLS, Gomez RA. The reninness score: integrative analysis of multi-omic data to define renin cell identity. 36th Annual Child Health Research Symposium, UVA. Poster presentation. 3rd Place Winner. May 2024.
Bingjie Xue, Silvia Medrano, Jason Smith, Alexandre Martini, Nathan Sheffield, Maria Luisa Sequeira Lopez, R. Ariel Gomez. The reninness score: integrative analysis of multi-omic data to define renin cell identity. AHA Council on Hypertension Meeting, Chicago, IL. Oral presentation. September 2024.
Ewelina Hejenkowska, Graduate Student, 2nd year: passed the Qualifying Exam and became PhD Candidate, 05/31/24. Mentor: Agnieszka Swiatecka-Urban MD,
Postdocs:
Jason Smith, PhD. Mentors: R. Ariel Gomez MD and Maria Luisa S. Sequeira-Lopez MD, UVA Pediatrics.
Dr. Smith is a Senior Research Scientist dedicated to the bioinformatic analysis of scRNA-seq, sc-ATAc-seq, proteomics, metabolomics, and lipidomics data from murine and human samples to better understand renin cell identity and nephrovascular development in health and disease. He has published several papers, and presented his work at meetings and symposia.
Publications
Martini AG, Smith JP, Medrano S, Sheffield NC, Sequeira-Lopez MLS, Gomez RA. Determinants of renin cell differentiation: a single cell epi-transcriptomics approach. bioRxiv [Preprint]. 2023 Jan 20:2023.01.18.524595. doi: 10.1101/2023.01.18.524595. PMID: 36711565; PMCID: PMC9882312.
Martini AG, Smith JP, Medrano S, Finer G, Sheffield NC, Sequeira-Lopez MLS, Gomez RA. Renin Cell Development: Insights From Chromatin Accessibility and Single-Cell Transcriptomics. Circ Res. 2023 Aug 4;133(4):369-371. doi: 10.1161/CIRCRESAHA.123.322827. Epub 2023 Jul 3. PMID: 37395102; PMCID: PMC10529662.
Jason P. Smith, Robert Paxton, Silvia Medrano, Nathan C. Sheffield, Maria Luisa S. Sequeira-Lopez, R. Ariel Gomez. Protein Kinase A Inhibition Epigenetically Silences Ren1. bioRxiv 2023.09.19.558267; doi: https://doi.org/10.1101/2023.09.19.558267
Smith JP, Sequeira-Lopez MLS, Gomez RA. A novel role for the histone modifier PRDM6 and an opportunity to understand hypertension. Kidney Int. 2023 Sep;104(3):415-417. doi: 10.1016/j.kint.2023.05.012. Epub 2023 May 24. PMID: 37236422; PMCID: PMC10529860.
Smith JP, Paxton R, Medrano S, Sheffield NC, Sequeira-Lopez MLS, Gomez RA. Inhibition of Renin Expression Is Regulated by an Epigenetic Switch From an Active to a Poised State. Hypertension. 2024 Sep;81(9):1869-1882. doi: 10.1161/HYPERTENSIONAHA.124.22886. Epub 2024 Jul 11. PMID: 38989586; PMCID: PMC11337216.
Medrano S, Yamaguchi M, Almeida LF, Smith JP, Yamaguchi H, Sigmund CD, Sequeira-Lopez MLS, Gomez RA. An efficient inducible model for the control of gene expression in renin cells. Am J Physiol Renal Physiol. 2024 Sep 1;327(3):F489-F503. doi: 10.1152/ajprenal.00129.2024. Epub 2024 Jul 11. PMID: 38991008.
Presentations
Xue B, Medrano S, Smith JP, Martini AG, Sheffield NC, Sequeira-Lopez MLS and Gomez RA. Specific Chromatin Configurations Across the Genome Determine the Renin Cell Identity. AHA Council on Hypertension Meeting. Poster presentation. September 2022, https://doi.org/10.1161/hyp.79.suppl_1.P116 Hypertension 2022.
Smith JP, Martini AG, Sheffield NC, Sequeira-Lopez MLS and Gomez RA. Epigenetic Determinants of Renin Cell Identity. AHA Council on Hypertension Meeting. Oral presentation. September 2022. https://www.ahajournals.org/doi/10.1161/hyp.79.suppl_1.011 September 2022.
Martini AG, Smith JP, Medrano S, Sheffield NC, Sequeira-Lopez MLS and Gomez RA. Distinct and Evolving Chromatin States and Transcription Factors Determine the Development of the kidney Vasculature. AHA Council on Hypertension Meeting. Oral presentation. September 2022. https://doi.org/10.1161/hyp.79.suppl_1.072
Smith JP, Martini AG, Sheffield NC, Sequeira-Lopez MLS and Gomez RA. Epigenetic Determinants of Renin Cell Identity. AHA Scientific Sessions. Oral presentation. November 2022.
Yamaguchi H, Smith JP, Li M, Watanabe H, Medrano S, Gomez RA and Sequeira-Lopez MLS. The renin cell baroreceptor: by single-cell RNA sequencing of renin lineage cells and in vitro/in vivo gene knockout experiments. 35th Annual Child Heath Research Symposium, UVA. Poster presentation. May 2023.
Smith JP, Nagalakshmi VK, Li M, Gomez RA and Sequeira Lopez MLS. Transcriptomic and Epigenomic Profile of a Mouse Kidney Aortic Coarctation Model. Annual Child Heath Research Trainee Competition, UVA. Oral presentation. 2nd Place Winner. May 2023.
Smith JP, Nagalakshmi VK, Li M, Gomez RA and Sequeira-Lopez MLS. Transcriptomic and Epigenomic Profile of a Mouse Kidney Aortic Coarctation Model. 35th Annual Child Heath Research Symposium, UVA. Oral presentation. May 2023.
Xue B, Medrano S, Smith JP, Martini AG, Sheffield NC, Sequeira-Lopez MLS, and Gomez RA. The Reninness Score: Integrative Analysis of Multi-Omic Data to Define Renin Cell Identity. 35th Annual Child Heath Research Symposium, UVA. Poster presentation. May 2023.
Almeida LF, Martini AG, Smith JP, Yamaguchi M, Medrano S, Sequeira-Lopez MLS, and Gomez RA. Multiomics Approach To Unravel The Molecular Mechanisms Responsible For Kidney Concentric Arterial And Arteriolar Hypertrophy. AHA Council on Hypertension Meeting, Boston, MA. Poster presentation. September 2023. https://doi.org/10.1161/hyp.80.suppl_1.P216.
Xue B, Medrano S, Smith J, Martini A, Sheffield N, Sequeira-Lopez MLS, Gomez RA.The reninness score: integrative analysis of multi-omic data to define renin cell identity. AHA Council on Hypertension Meeting, Boston, MA. Poster presentation. September 2023. https://doi.org/10.1161/hyp.80.suppl_1.P225.
Yamaguchi H, Smith JP, Li M, Watanabe H, Medrano S, Gomez RA, and Sequeira-Lopez MLS. Klf2, A Transcriptional Component Of The Renin Cell Baroreceptor. AHA Council on Hypertension Meeting, Boston, MA. Oral presentation. September 2023. https://www.ahajournals.org/doi/abs/10.1161/hyp.80.suppl_1.069.
Smith JP, Paxton R, Sequeira-Lopez MLS, and Gomez RA. Protein Kinase A Inhibition Epigenetically Silences Ren1. AHA Council on Hypertension Meeting, Boston, MA. Poster presentation. September 2023. https://www.ahajournals.org/doi/10.1161/hyp.80.suppl_1.P153.
Vidya K. Nagalakshmi, Jason P. Smith, Minghong Li, Hirofumi Watanabe, Silvia Medrano, Ariel Gomez, Maria Luisa S. Sequeira-Lopez. Renin Cell Identity And Blood Pressure Homeostasis: Exploring The Role Of Endothelial Cell Cross-talk. AHA Council on Hypertension Meeting, Boston, MA. Oral presentation. September 2023. http://dx.doi.org/10.1161/hyp.80.suppl_1.124.
Yamaguchi H, Smith JP, Li M, Watanabe H, Medrano S, Gomez RA, and Sequeira-Lopez MLS. Klf2, A Transcriptional Component Of The Renin Cell Baroreceptor. AHA Scientific Sessions. Philadelphia, PA. Poster presentation. November 2023.
Gomez RA, Medrano S and Smith JP. UVA PCEN. Pediatric Center of Excellence in Nephrology (PCEN) Network Symposium. Oral presentation. February 2024.
Almeida LF, Medrano S, Smith JP, Yamaguchi M, Liang X, Sequeira-Lopez MLS, Gomez RA. Proteomics and Integrative “Omics” Strategies to Decipher the pathways responsible for Concentric Hypertrophy of the renal arteries and arterioles. 36th Annual Child Health Research Trainee Competition and Symposium, UVA. Oral and poster presentation. 2nd Place Winner (LFA, poster presentation). May 2024.
Smith JP, Yamaguchi M, Gomez RA, Sequeira-Lopez MLS. Recruitment Increases Juxtaglomerular Cell Signaling Within Collagen, APP, SPP1, Laminin, THBS, VEGF, and GAS Pathways. 36th Annual Child Health Research Symposium, UVA. Poster presentation. May 2024.
Yamaguchi M, Sinharoy A, Yamaguchi H, Almeida LF, Liang X, Smith JP, Medrano S, Sequeira-Lopez MLS, Gomez RA. Hyperinnervation of renin cells and renal arterial tree during long – term inhibition of the renin-angiotensin system (RAS). 36th Annual Child Health Research Trainee Competition and Symposium, UVA. Oral and poster presentation. 4th Place Winner (MY, oral presentation). May 2024.
Xue B, Medrano S, Smith JP, Martini A, Sheffield NC, Sequeira-Lopez MLS, Gomez RA. The reninness score: integrative analysis of multi-omic data to define renin cell identity. 36th Annual Child Health Research Symposium, UVA. Poster presentation. 3rd Place Winner (BX). May 2024.
Jason P. Smith, Manako Yamaguchi, R. Ariel Gomez, Maria Luisa S. Sequeira Lopez. Spatial Transcriptomics Reveals How Smooth Muscle Cells Transform to Renin-Expressing Cells During Homeostatic Challenge. AHA Council on Hypertension Meeting, Chicago, IL. Oral presentation. September 2024. Winner of Trainee Advocacy Committee New Investigator Travel Award.
Lucas Almeida, Silvia Medrano, Jason P. Smith, Manako Yamaguchi, Ariel Gomez, Maria Luisa S. Sequeira-Lopez. Identifying markers of Concentric Arterial and Arteriolar Hypertrophy (CAAH) using multiomics. AHA Council on Hypertension Meeting, Chicago, IL. Poster presentation. September 2024.
Vidya K. Nagalakshmi, Daisuke Matsuoka, Alexandre Martini, Jason P. Smith, Ariel Gomez, Maria Luisa S. Sequeira-Lopez. Piezo2 Channels in JG cells do not Regulate Renin Expression or Renin Release to the Circulation. AHA Council on Hypertension Meeting, Chicago, IL. Poster presentation. September 2024.
Manako Yamaguchi, Lucas Ferreira de Almeida, Hiroki Yamaguchi, Jason P. Smith, Silvia Medrano, Maria Luisa Sequeira Lopez, R. Ariel Gomez. Hyperinnervation and Immune-endocrine Interactions in Renal Arteriolar Hypertrophy during Inhibition of The Renin-Angiotensin System. AHA Council on Hypertension Meeting, Chicago, IL. Oral presentation. September 2024.
Hiroki Yamaguchi, MD, PhD. Mentors: R. Ariel Gomez MD and Maria Luisa S. Sequeira-Lopez MD.
Dr. Yamaguchi is a Research Associate in the Gomez/Sequeira-Lopez labs. He is working on the renin cell baroreptor.
Publications
Yamaguchi H, Gomez RA, Sequeira-Lopez MLS. Renin Cells, From Vascular Development to Blood Pressure Sensing. Hypertension. 2023 Jun 14. doi: 10.1161/HYPERTENSIONAHA.123.20577. Epub ahead of print. PMID: 37313725
Silvia Medrano, Manako Yamaguchi, Hiroki Yamaguchi, Lucas Ferreira de Almeida, Curt D. Sigmund, Maria Luisa S. Sequeira-Lopez, R. Ariel Gomez. An efficient inducible model for the control of gene expression in renin cells bioRxiv 2024.03.26.585202; doi: https://doi.org/10.1101/2024.03.26.585202.
Medrano S, Yamaguchi M, Almeida LF, Smith JP, Yamaguchi H, Sigmund CD, Sequeira-Lopez MLS, Gomez RA. An efficient inducible model for the control of gene expression in renin cells. Am J Physiol Renal Physiol. 2024 Sep 1;327(3):F489-F503. doi: 10.1152/ajprenal.00129.2024. Epub 2024 Jul 11. PMID: 38991008.
Presentations
Yamaguchi H, Smith JP, Li M, Watanabe H, Medrano S, Gomez RA and Sequeira-Lopez MLS. The renin cell baroreceptor: by single-cell RNA sequencing of renin lineage cells and in vitro/in vivo gene knockout experiments. 35th Annual Child Heath Research Symposium, UVA. Poster presentation. May 2023.
Yamaguchi M, Yamaguchi H, Medrano S, Tainaka K, Sequeira-Lopez MLS and Gomez RA. A method of 3D visualization of specific cell types and structures during kidney vascular development. 35th Annual Child Heath Research Symposium, UVA. Poster presentation. May 2023.
Yamaguchi M, Yamaguchi H, Medrano S, Tainaka K, Sequeira-Lopez MLS, and Gomez RA. Three-dimensional Visualization And Evaluation Of Differences In Renin Expression In Kidneys Exposed In Vivo To Either Low Or High Arterial Pressure. AHA Council on Hypertension Meeting, Boston, MA. Oral presentation. September 2023. https://www.ahajournals.org/doi/10.1161/hyp.80.suppl_1.026.
Yamaguchi H, Smith JP, Li M, Watanabe H, Medrano S, Gomez RA, and Sequeira-Lopez MLS. Klf2, A Transcriptional Component Of The Renin Cell Baroreceptor. AHA Council on Hypertension Meeting, Boston, MA. Oral presentation. September 2023. https://www.ahajournals.org/doi/abs/10.1161/hyp.80.suppl_1.069.
Hiroki Yamaguchi, Manako Yamaguchi, Hirofumi Watanabe, Silvia Medrano, Maria Luisa S. Sequeira Lopez, R. Ariel Gomez. Cellular level transcriptomics and three-dimensional imaging for recruited renin cells. American Society of Nephrology Kidney Week 2023, Philadelphia, TH-OR83, 2023 (Oral presentation).
Yamaguchi H, Smith JP, Li M, Watanabe H, Medrano S, Gomez RA, and Sequeira-Lopez MLS. Klf2, A Transcriptional Component Of The Renin Cell Baroreceptor. AHA Scientific Sessions. Philadelphia, PA. Poster presentation. November 2023.
Yamaguchi H, Guagliardo NA, Medrano S, Gomez RA, Sequeira-Lopez MLS. Angiotensin II Suppresses renin release by eliciting robust calcium oscillations in juxtaglomerular cells. 36th Annual Child Health Research Trainee Competition and Symposium, UVA. Oral and poster presentation. 3rd Place Winner (oral presentation). May 2024.
Yamaguchi M, Sinharoy A, Yamaguchi H, Almeida LF, Liang X, Smith JP, Medrano S, Sequeira-Lopez MLS, Gomez RA. Hyperinnervation of renin cells and renal arterial tree during long – term inhibition of the renin-angiotensin system (RAS). 36th Annual Child Health Research Trainee Competition and Symposium, UVA. Oral and poster presentation. 2nd Place Winner YM (oral presentation). May 2024.
Ahana Sinharoy*, Hoang Yen Ngoc Le*, Manako Yamaguchi MD PhD, Lucas Ferreira de Almeida MS PhD, Hiroki Yamaguchi MD PhD, Silvia Medrano PhD, Maria Luisa S. Sequeira-Lopez MD PhD, R. Ariel Gomez MD PhD * Equal contribution. Three-Dimensional Visualization of Concentric Renal Arteriolar Hypertrophy in Renin Gene Knockout Mouse. KUH Summer Undergraduate Research Symposium, July31-August 2, 2024. University of Virginia.
Manako Yamaguchi, Lucas Ferreira de Almeida, Hiroki Yamaguchi, Jason P. Smith, Silvia Medrano, Maria Luisa Sequeira Lopez, R. Ariel Gomez. Hyperinnervation and Immune-endocrine Interactions in Renal Arteriolar Hypertrophy during Inhibition of The Renin-Angiotensin System. AHA Council on Hypertension Meeting, Chicago, IL. Oral presentation. September 2024.
Hiroki Yamaguchi, Nick A. Guagliardo, Silvia Medrano, R. Ariel Gomez, and Maria Luisa S. Sequeira-Lopez. Angiotensin II Inhibits Renin Secretion Directly By Eliciting Robust Calcium Oscillations In Juxtaglomerular Cells. AHA Council on Hypertension Meeting, Chicago, IL. Oral presentation. September 2024.
Manako Yamaguchi, MD, PhD. Mentors: R. Ariel Gomez MD and Maria Luisa S. Sequeira-Lopez MD, UVA Pediatrics.
Dr. Yamaguchi is a Research Associate in the Gomez/Sequeira-Lopez labs. She is conducting spatial transcriptome analysis of mouse kidneys under normal and pathological conditions, and different developmental stages to detect changes in renin cell distribution and RNA expression profiles during development. She is also studying innervation in the kidney vasculature.
Publications
Silvia Medrano, Manako Yamaguchi, Hiroki Yamaguchi, Lucas Ferreira de Almeida, Curt D. Sigmund, Maria Luisa S. Sequeira-Lopez, R. Ariel Gomez. An efficient inducible model for the control of gene expression in renin cells bioRxiv 2024.03.26.585202; doi: https://doi.org/10.1101/2024.03.26.585202
Medrano S, Yamaguchi M, Almeida LF, Smith JP, Yamaguchi H, Sigmund CD, Sequeira-Lopez MLS, Gomez RA. An efficient inducible model for the control of gene expression in renin cells. Am J Physiol Renal Physiol. 2024 Sep 1;327(3):F489-F503. doi: 10.1152/ajprenal.00129.2024. Epub 2024 Jul 11. PMID: 38991008.
Presentations
Yamaguchi M, Yamaguchi H, Medrano S, Tainaka K, Sequeira-Lopez MLS and Gomez RA. A method of 3D visualization of specific cell types and structures during kidney vascular development. 35th Annual Child Heath Research Symposium, UVA. Poster presentation. May 2023.
Yamaguchi M, Yamaguchi H, Medrano S, Tainaka K, Sequeira-Lopez MLS, and Gomez RA. Three-dimensional Visualization And Evaluation Of Differences In Renin Expression In Kidneys Exposed In Vivo To Either Low Or High Arterial Pressure. AHA Council on Hypertension Meeting, Boston, MA. Oral presentation. September 2023. https://www.ahajournals.org/doi/10.1161/hyp.80.suppl_1.026.
Almeida LF, Martini AG, Smith JP, Yamaguchi M, Medrano S, Sequeira-Lopez MLS, and Gomez RA. Multiomics Approach To Unravel The Molecular Mechanisms Responsible For Kidney Concentric Arterial And Arteriolar Hypertrophy. AHA Council on Hypertension Meeting, Boston, MA. Poster presentation. September 2023. https://doi.org/10.1161/hyp.80.suppl_1.P216.
Hiroki Yamaguchi, Manako Yamaguchi, Hirofumi Watanabe, Silvia Medrano, Maria Luisa S. Sequeira Lopez, R. Ariel Gomez. Cellular level transcriptomics and three-dimensional imaging for recruited renin cells. American Society of Nephrology Kidney Week 2023, Philadelphia, TH-OR83, 2023 (Oral presentation).
Almeida LF, Medrano S, Smith JP, Yamaguchi M, Liang X, Sequeira-Lopez MLS, Gomez RA. Proteomics and Integrative “Omics” Strategies to Decipher the pathways responsible for Concentric Hypertrophy of the renal arteries and arterioles. 36th Annual Child Health Research Trainee Competition and Symposium, UVA. Oral and poster presentation. May 2024.
Smith JP, Yamaguchi M, Gomez RA, Sequeira-Lopez MLS. Recruitment Increases Juxtaglomerular Cell Signaling Within Collagen, APP, SPP1, Laminin, THBS, VEGF, and GAS Pathways. 36th Annual Child Health Research Symposium, UVA. Poster presentation. May 2024.
Yamaguchi M, Sinharoy A, Yamaguchi H, Almeida LF, Liang X, Smith JP, Medrano S, Sequeira-Lopez MLS, Gomez RA. Hyperinnervation of renin cells and renal arterial tree during long – term inhibition of the renin-angiotensin system (RAS). 36th Annual Child Health Research Trainee Competition and Symposium, UVA. Oral and poster presentation. 4th Place Winner (oral). May 2024.
Ahana Sinharoy*, Hoang Yen Ngoc Le*, Manako Yamaguchi MD PhD, Lucas Ferreira de Almeida MS PhD, Hiroki Yamaguchi MD PhD, Silvia Medrano PhD, Maria Luisa S. Sequeira Lopez MD PhD, R. Ariel Gomez MD PhD * Equal contribution. Three-Dimensional Visualization of Concentric Renal Arteriolar Hypertrophy in Renin Gene Knockout Mouse. KUH Summer Undergraduate Research Symposium, July31-August 2, 2024. University of Virginia.
Lucas Almeida, Silvia Medrano, Jason P. Smith, Manako Yamaguchi, Ariel Gomez, Maria Luisa S. Sequeira-Lopez. Identifying markers of Concentric Arterial and Arteriolar Hypertrophy (CAAH) using multiomics. AHA Council on Hypertension Meeting, Chicago, IL. Poster presentation. September 2024
Jason P. Smith, Manako Yamaguchi, R. Ariel Gomez, Maria Luisa S. Sequeira-Lopez. Spatial Transcriptomics Reveals How Smooth Muscle Cells Transform to Renin-Expressing Cells During Homeostatic Challenge. AHA Council on Hypertension Meeting, Chicago, IL. Oral presentation. September 2024.
Manako Yamaguchi, Lucas Ferreira de Almeida, Hiroki Yamaguchi, Jason P. Smith, Silvia Medrano, Maria Luisa Sequeira Lopez, R. Ariel Gomez. Hyperinnervation and Immune-endocrine Interactions in Renal Arteriolar Hypertrophy during Inhibition of The Renin-Angiotensin System. AHA Council on Hypertension Meeting, Chicago, IL. Oral presentation. September 2024. Winner of New Investigator Award for Japanese Fellows at Hypertension 2024.
Lucas Almeida, PhD. Mentors: R. Ariel Gomez MD and Maria Luisa S. Sequeira-Lopez MD
Dr. Almeida is a Research Associate in the Gomez/ Sequeira-Lopez labs. He is studying the mechanisms involved in RAS inhibition-induced concentric vascular hypertrophy (PCEN Project 1).
Publications
Silvia Medrano, Manako Yamaguchi, Hiroki Yamaguchi, Lucas Ferreira de Almeida, Curt D. Sigmund, Maria Luisa S. Sequeira-Lopez, R. Ariel Gomez. An efficient inducible model for the control of gene expression in renin cells bioRxiv 2024.03.26.585202; doi: https://doi.org/10.1101/2024.03.26.585202
Medrano S, Yamaguchi M, Almeida LF, Smith JP, Yamaguchi H, Sigmund CD, Sequeira-Lopez MLS, Gomez RA. An efficient inducible model for the control of gene expression in renin cells. Am J Physiol Renal Physiol. 2024 Sep 1;327(3):F489-F503. doi: 10.1152/ajprenal.00129.2024. Epub 2024 Jul 11. PMID: 38991008.
Almeida LF, Ariel Gomez R, Sequeira-Lopez MLS. Elusive and Heterogenous Nature of Renin Cells. Hypertension. 2024 Feb;81(2):203-205. doi: 10.1161/HYPERTENSIONAHA.123.21372. Epub 2023 Sep 26. PMID: 37750306; PMCID: PMC10840598.
Presentations
Almeida LF, Martini AG, Medrano S, Sequeira-Lopez MLS, and Gomez RA. Multiomics Approach to Unravel the Molecular Mechanisms Responsible for Kidney Concentric Arterial and Arteriolar Hypertrophy. 35th Annual Child Heath Research Symposium, UVA. Poster presentation. Trainee competition Second place winner. May 2023.
Almeida LF, Martini AG, Smith JP, Yamaguchi M, Medrano S, Sequeira-Lopez MLS, and Gomez RA. Multiomics Approach To Unravel The Molecular Mechanisms Responsible For Kidney Concentric Arterial And Arteriolar Hypertrophy. AHA Council on Hypertension Meeting, Boston, MA. Poster presentation. September 2023. https://doi.org/10.1161/hyp.80.suppl_1.P216.
Almeida LF, Medrano S, Smith JP, Yamaguchi M, Liang X, Sequeira-Lopez MLS, Gomez RA. Proteomics and Integrative “Omics” Strategies to Decipher the pathways responsible for Concentric Hypertrophy of the renal arteries and arterioles. 36th Annual Child Health Research Trainee Competition and Symposium, UVA. Oral and poster presentation. 2nd Place Winner (poster presentation). May 2024.
Yamaguchi M, Sinharoy A, Yamaguchi H, Almeida LF, Liang X, Smith JP, Medrano S, Sequeira-Lopez MLS, Gomez RA. Hyperinnervation of renin cells and renal arterial tree during long – term inhibition of the renin-angiotensin system (RAS). 36th Annual Child Health Research Trainee Competition and Symposium, UVA. Oral and poster presentation.
Medrano S, Almeida LF, Sequeira-Lopez MLS, Gomez RA. A New Mouse Model of RAS Deficiency-Induced Kidney Concentric Vascular Hypertrophy. 36th Annual Child Health Research Symposium, UVA. Poster presentation. May 2024.
Ahana Sinharoy*, Hoang Yen Ngoc Le*, Manako Yamaguchi MD PhD, Lucas Ferreira de Almeida MS PhD, Hiroki Yamaguchi MD PhD, Silvia Medrano PhD, Maria Luisa S. Sequeira Lopez MD PhD, R. Ariel Gomez MD PhD * Equal contribution. Three-Dimensional Visualization of Concentric Renal Arteriolar Hypertrophy in Renin Gene Knockout Mouse. KUH Summer Undergraduate Research Symposium, July 31-August 2, 2024. University of Virginia.
Gigi Toirova*, Arianna Romero-Gonzalez*, Lucas Ferreira de Almeida MS PhD, Silvia Medrano PhD, Xuiyin Liang, Maria Luisa S. Sequeira-Lopez MD, R. Ariel Gomez MD * Equal contribution. Reversibility of Acute Renin Cell Recruitment in the Mouse. KUH Summer Undergraduate Research Symposium, July 31-August 2, 2024. University of Virginia.
Lucas Almeida, Silvia Medrano, Jason P. Smith, Manako Yamaguchi, Ariel Gomez, Maria Luisa S. Sequeira-Lopez. Identifying markers of Concentric Arterial and Arteriolar Hypertrophy (CAAH) using multiomics. AHA Council on Hypertension Meeting, Chicago, IL. Poster presentation. September 2024.
Manako Yamaguchi, Lucas Ferreira de Almeida, Hiroki Yamaguchi, Jason P. Smith, Silvia Medrano, Maria Luisa Sequeira Lopez, R. Ariel Gomez. Hyperinnervation and Immune-endocrine Interactions in Renal Arteriolar Hypertrophy during Inhibition of The Renin-Angiotensin System. AHA Council on Hypertension Meeting, Chicago, IL. Oral presentation. September 2024.
Silvia Medrano, Lucas F. Almeida, Maria Luisa S. Sequeira-Lopez and R. Ariel Gomez. Impaired Renin Activity in a Bicistronic Ren1c-T2A-tdTomato Reporter Mouse. AHA Council on Hypertension Meeting, Chicago, IL. Poster presentation. September 2024.
Daisuke Matsuoka MD, PhD. Mentors: R. Ariel Gomez MD and Maria Luisa S. Sequeira-Lopez MD, UVA Pediatrics.
D.r Matsuoka is a Research Associate in thr Gomez /Sequeira-Lopez labs. He is working on the renin cell baroreptor.
Presentations
Matsuoka D, Gomez RA, Sequeira-Lopez MLS. Establishment of mouse surgical models to elucidate the acute renin baroreceptor mechanism. 36th Annual Child Health Research Symposium, UVA. Poster presentation. May 2024.
Vidya K. Nagalakshmi, Daisuke Matsuoka, Alexandre Martini, Jason P. Smith, Ariel Gomez, Maria Luisa S. Sequeira-Lopez. Piezo2 Channels in JG cells do not Regulate Renin Expression or Renin Release to the Circulation. AHA Council on Hypertension Meeting, Chicago, IL. Poster presentation. September 2024
Hayrettin Yavuz, MD, PhD. Postdoctoral Fellow in Urban Lab. Mentor: Agnieszka Swiatecka-Urban. Project: Kidney Function in PwCF in the Era of HEMT
Junior Faculty:
Vidya Nagalakshmi, PhD, Instructor of Research.
Dr. Nagalakshmi worked on the role of renin cells during regeneration after UUO release and the Renin baroreceptor. She is working on the role of Piezo channels in renin cells.
Publications
Nagalakshmi VK, Li M, Liang X, Medrano S, Belyea BC, Gomez RA, Sequeira-Lopez MLS. Cells of the renin lineage promote kidney regeneration post-release of ureteral obstruction in neonatal mice. Acta Physiol (Oxf). 2023 Aug;238(4):e14014. doi: 10.1111/apha.14014. Epub 2023 Jun 19. PMID: 37309075; PMCID: PMC10527043.
Presentations
Nagalakshmi VK, Li M, Medrano S, Gomez RA and Sequeira-Lopez MLS. Cells of the Renin Lineage Promote Kidney Regeneration Post-Release of Ureteral Obstruction in Neonatal Mice. Oral presentation at Council on Hypertension Meeting, San Diego, CA. September 2022.
Nagalakshmi VK, Li M, Gomez RA and Sequeira-Lopez MLS. Renin Cell Identity and Blood Pressure Homeostasis: Exploring the Role of Endothelial Cell Cross-Talk. 35th Annual Child Heath Research Symposium, UVA. Poster presentation. May 2023.
Nagalakshmi VK, Li M, Gomez RA and Sequeira-Lopez MLS. Endothelial Cell Fate During Kidney Regeneration Post-Release of Ureteral Obstruction: Lineage Tracing and Transcriptomics Approach. 35th Annual Child Heath Research Symposium, UVA. Poster presentation. May 2023.
Vidya K. Nagalakshmi, Jason P. Smith, Minghong Li, Hirofumi Watanabe, Silvia Medrano, Ariel Gomez, Maria Luisa S. Sequeira-Lopez. Renin Cell Identity And Blood Pressure Homeostasis: Exploring The Role Of Endothelial Cell Cross-talk. AHA Council on Hypertension Meeting, Boston, MA. Oral presentation. September 2023. http://dx.doi.org/10.1161/hyp.80.suppl_1.124.
Nagalakshmi VK, Daisuke Matsuoka, Sequeira-Lopez MLS, Gomez RA. Piezo2 Channels in JG cells do not Regulate Renin Expression or Renin Release to the Circulation. 36th Annual Child Health Research Symposium, UVA. Poster presentation. May 2024.