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Members of the Sheynkman lab are underlined.

Characterization of a COPD-Associated Nephronectin Functional Splicing Genetic Variant in Human Lung Tissue via Long-Read Sequencing

Aabida Saferali, Anastacia N. Wienecke, Zhonghui Xu, Tao Liu, Gloria M. Sheynkman, Craig P. Hersh, Michael H. Cho, Edwin K. Silverman, Xiaobo Zhou, Carole L. Wilson, Lynn M. Schnapp, Scott H. Randell, Silvia B. V. Ramos, Alain Laederach, Christopher Vollmers, Peter J. Castaldi. 2025. European Respiratory Journal.

 

IS-PRM-based peptide targeting informed by long-read sequencing for alternative proteome detection

Jennifer A. KorchakErin D. JefferySaikat BandyopadhyayBen T. JordanMicah D. LeheEmily F. Watts, Aidan Fenix, Mathias Wilhelm, Gloria M. Sheynkman. 2024. Journal of the American Society for Mass Spectrometry

AltportDet Graph

Aboud etal Fig

Long-read proteogenomics to connect disease-associated sQTLs to 2 the protein isoform effectors of disease

Abdullah Abood, Larry D. Mesner, Erin D. Jeffery , Mayank Murali , Micah D. Lehe , Jamie Saquing , Charles R. Farber, Gloria M. Sheynkman. The American Journal of Human Genetics. 2024. 111(9): 1914-1931.

Biosurfer for systematic tracking of regulatory mechanisms leading to protein isoform diversity

Murali M, Saquing J, Lu S, Gao Z, Jordan B, Wakefield ZP, Fiszbein A, Cooper DR, Castaldi PJ, Korkin D, Sheynkman G. Biosurfer for systematic tracking of regulatory mechanisms leading to protein isoform diversity. bioRxiv [Preprint]. 2024 Mar

Systematic assessment of long-read RNA-seq methods for transcript identification and quantification

F.J. Pardo-Palacios, D. Wang, F. Reese, M. Diekhans, S. Carbonell-Sala, B. Williams, J.E. Loveland, M. De María, M.S. Adams, G. Balderrama-Gutierrez, A.K. Behera, J.M. Gonzalez, T. Hunt, J. Lagarde, C.E. Liang, H. Li, M. Jerryd Meade, D.A. Moraga Amador, A.D. Prjibelski, I. Birol, H. Bostan, A.M. Brooks, M. Hasan Çelik, Y. Chen, M.R.M. Du, C. Felton, J. Göke, S. Hafezqorani, R. Herwig, H. Kawaji, J. Lee, J. Liang Li, M. Lienhard, A. Mikheenko, D. Mulligan, K. Ming Nip, M. Pertea, M.E. Ritchie, A.D. Sim, A.D. Tang, Y. Kei Wan, C. Wang, B.Y. Wong, C. Yang, I. Barnes, A. Berry, S. Capella, N. Dhillon, J.M. Fernandez-Gonzalez, L. Ferrández-Peral, N. Garcia-Reyero, S. Goetz, C. Hernández-Ferrer, L. Kondratova, T. Liu, A. Martinez-Martin, C. Menor, J. Mestre-Tomás, J.M. Mudge, N.G. Panayotova, A. Paniagua, D. Repchevsky, E. Rouchka, B. Saint-John, E. Sapena, Leon Sheynkman, M. Laird Smith, M.M. Suner, H. Takahashi, I.A. Youngworth, P. Carninci, N.D. Denslow, R. Guigó, M.E. Hunter, H.U. Tilgner, B.J. Wold, C. Vollmers, A. Frankish, K. Fai Au, Gloria M. Sheynkman, A. Mortazavi, A. Conesa, A.N. Brooks. Nature Methods. 2024. 21: 1349-1363.

LRGASP graph

HUVEC paper

Characterization of protein isoform diversity in human umbilical vein endothelial cells via long-read proteogenomics

Madison M. MehlferberBen T. JordanErin D. JefferyLeon SheynkmanJamie SaquingBipul R. AcharyaKaren K. HirschiGloria M. Sheynkman. RNA Biology. 2022. 19(1): 1228-1243.

Enhanced protein isoform characterization through long-read proteogenomics

Rachel M. Miller, Ben T. Jordan, Madison M. Mehlferber, Erin D. Jeffery, Christina Chatzipantsiou, Simi Kaur, Robert J. Millikin, Yunxiang Dai, Simone Tiberi, Peter J. Castaldi, Michael R. Shortreed, Chance John Luckey, Ana Conesa, Lloyd M. Smith, Anne Deslattes Mays, Gloria M. Sheynkman. Genome Biology. 2022. 23(1): 69.

LRP pipeline

Brain long reads

Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing

Szi Kay Leung, Aaron R. Jeffries, Isabel Castanho, Ben T. Jordan, Karen Moore, Jonathan P. Davies, Emma L. Dempster, Nicholas J. Bray, Paul O’Neill, Elizabeth Tseng, Zeshan Ahmed, David A. Collier, Erin D. Jeffery, Shyam Prabhakar, Leonard Schalkwyk, Connor Jops, Michael J. Gandal, Gloria M. Sheynkman, Elis Hannon, Jonathan Mill. Cell Reports. 2021. 37(7): 110022.

Interpretable artificial intelligence and exascale molecular dynamics simulations to reveal kinetics: Applications to Alzheimer’s disease

William Martin, Gloria Sheynkman, Felice C. Lightstone, Ruth Nussinov, Feixiong Cheng. Current Opinion in Structural Biology. 2022. 72: 103-113.

Martin et al. 2022 Figure 3

Saferali et al. 2020 Fig 1

Characterization of a COPD-Associated NPNT Functional Splicing Genetic Variant in Human Lung Tissue via Long-Read Sequencing

Aabida SaferaliZhonghui XuGloria M. Sheynkman, Craig P. Hersh, Michael H. ChoEdwin K. SilvermanAlain LaederachChristopher VollmersPeter J. Castaldi. medRxiv [Preprint]. 2020. 2020.10.20.20203927.

 

Human PPIs

A reference map of the human binary protein interactome

Katja Luck, Dae-Kyum Kim, Luke Lambourne, Kerstin Spirohn, Bridget E. Begg, Wenting Bian, Ruth Brignall, Tiziana Cafarelli, Francisco J. Campos-Laborie, Benoit Charloteaux, Dongsic Choi, Atina G. Coté, Meaghan Daley, Steven Deimling, Alice Desbuleux, Amélie Dricot, Marinella Gebbia, Madeleine F. Hardy, Nishka Kishore, Jennifer J. Knapp, István A. Kovács, Irma Lemmens, Miles W. Mee, Joseph C. Mellor, Carl Pollis, Carles Pons, Aaron D. Richardson, Sadie Schlabach, Bridget Teeking, Anupama Yadav, Mariana Babor, Dawit Balcha, Omer Basha, Christian Bowman-Colin, Suet-Feung Chin, Soon Gang Choi, Claudia Colabella, Georges Coppin, Cassandra D’Amata, David De Ridder, Steffi De Rouck, Miquel Duran-Frigola, Hanane Ennajdaoui, Florian Goebels, Liana Goehring, Anjali Gopal, Ghazal Haddad, Elodie Hatchi, Mohamed Helmy, Yves Jacob, Yoseph Kassa, Serena Landini, Roujia Li, Natascha van Lieshout, Andrew MacWilliams, Dylan Markey, Joseph N. Paulson, Sudharshan Rangarajan, John Rasla, Ashyad Rayhan, Thomas Rolland, Adriana San-Miguel, Yun Shen, Dayag Sheykhkarimli, Gloria M. Sheynkman, Eyal Simonovsky, Murat Taşan, Alexander Tejeda, Vincent Tropepe, Jean-Claude Twizere, Yang Wang, Robert J. Weatheritt, Jochen Weile, Yu Xia, Xinping Yang, Esti Yeger-Lotem, Quan Zhong, Patrick Aloy, Gary D. Bader, Javier De Las Rivas, Suzanne Gaudet, Tong Hao, Janusz Rak, Jan Tavernier, David E. Hill, Marc Vidal, Frederick P. Roth & Michael A. Calderwood. Nature. 2020. 580(7803): 402-408.

ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms

Gloria M. Sheynkman, Katharine S. Tuttle, Florent Laval, Elizabeth Tseng, Jason G. Underwood, Liang Yu, Da Dong, Melissa L. Smith, Robert Sebra, Luc Willems, Tong Hao, Michael A. Calderwood, David E. Hill & Marc Vidal . 2020. Nature communications. 11(1): 2326.

ORFCapture-Seq table

Schmidt et al. 2019. Fig 1

The lncRNA SLNCR Recruits the Androgen Receptor to EGR1-Bound Genes in Melanoma and Inhibits Expression of Tumor Suppressor

Karyn Schmidt, Johanna S. Carroll, Elaine Yee, Dolly D. Thomas, Leon Wert-Lamas, Steven C. Neier, Gloria Sheynkman, Justin Ritz, Carl D. Novina. 2019. Cell Reports. 27(8): 2493-2507.e4.

Miller et al. 2019. Abstract

Improved Protein Inference from Multiple Protease Bottom-Up Mass Spectrometry Data

Rachel M. Miller, Robert J. Millikin, Connor V. Hoffmann, Stefan K. Solntsev, Gloria M. Sheynkman, Michael R. Shortreed, Lloyd M. Smith. 2019. Journal of Proteome Research. 18(9): 3429-3438.

 Full-length mRNA sequencing uncovers a widespread coupling between transcription initiation and mRNA processing

Seyed Yahya Anvar, Guy Allard, Elizabeth Tseng, Gloria M. Sheynkman, Eleonora de Klerk, Martijn Vermaat, Raymund H. Yin, Hans E. Johansson, Yavuz Ariyurek, Johan T. den Dunnen, Stephen W. Turner & Peter A. C. ‘t Hoen. 2018. Genome biology. 19(1): 46.

Yahya et al. 2018 Fig 1

PPI mapping

Proteome-Scale Human Interactomics

Katja Luck, Gloria M. Sheynkman, Ivy Zhang, Marc Vidal. 2017. Trends in Biochemical Sciences. 42(5): 342-354.

Proteogenomics: Integrating Next-Generation Sequencing and Mass Spectrometry to Characterize Human Proteomic Variation

Gloria M. Sheynkman, Michael R. Shortreed, Anthony J. Cesnik, and Lloyd M. Smith. 2016; Annual review of analytical chemistry (Palo Alto, Calif.). 9(1): 521-45.

Sheynkman et al. 2016 Figure 1

Keller et al. 2016 Figure 2

The Transcription Factor Nfatc2 Regulates β-Cell Proliferation and Genes Associated with Type 2 Diabetes in Mouse and Human Islets

Mark P. Keller, Pradyut K. Paul, Mary E. Rabaglia, Donnie S. Stapleton, Kathryn L. Schueler, Aimee Teo Broman, Shuyun Isabella Ye, Ning Leng, Christopher J. Brandon, Elias Chaibub Neto, Christopher L. Plaisier, Shane P. Simonett, Melkam A. Kebede, Gloria M. Sheynkman, Mark A. Klein, Nitin S. Baliga, Lloyd M. Smith, Karl W. Broman, Brian S. Yandell, Christina Kendziorski, Alan D. Attie. 2016. PLoS Genetics. 12(12): e1006466.

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing

Xinping Yang, Jasmin Coulombe-Huntington, Shuli Kang, Gloria M. Sheynkman, Tong Hao, Aaron Richardson, Song Sun, Fan Yang, Yun A. Shen, Ryan R. Murray, Kerstin Spirohn, Bridget E. Begg, Miquel Duran-Frigola, Andrew MacWilliams, Samuel J. Pevzner, Quan Zhong, Shelly A. Wanamaker, Stanley Tam, Lila Ghamsari, Nidhi Sahni, Song Yi, Maria D. Rodriguez, Dawit Balcha, Guihong Tan, Michael Costanzo, Brenda Andrews, Charles Boone, Xianghong J. Zhou, Kourosh Salehi-Ashtiani, Benoit Charloteaux, Alyce A. Chen, Michael A. Calderwood, Patrick Aloy, Frederick P. Roth, David E. Hill, Lilia M. Iakoucheva, Yu Xia, Marc Vidal. 2016. Cell. 164(4): 805-17.

Isoform profiling

Shortreed et al. 2015 Abstract

Global Identification of Protein Post-translational Modifications in a Single-Pass Database Search

Michael R. Shortreed, Craig D. Wenger, Brian L. Frey, Gloria M. Sheynkman, Mark Scalf, Mark P. Keller, Alan D. Attie, Lloyd M. Smith. 2015; Journal of Proteome Research. 14(11): 4714-20.

Human Proteomic Variation Revealed by Combining RNA-Seq Proteogenomics and Global Post-Translational Modification (G-PTM) Search Strategy

Anthony J. Cesnik, Michael R. Shortreed, Gloria M. Sheynkman, Brian L. Frey, Lloyd M. Smith. 2015. Journal of Proteome Research. 15(3): 800-8.

Cesnik et al. 2015 Fig 1

Jagtap et al. 2014 Abstract

Flexible and accessible workflows for improved proteogenomic analysis using the Galaxy framework

Pratik D. Jagtap, James E. Johnson, Getiria Onsongo, Fredrik W. Sadler, Kevin Murray, Yuanbo Wang, Gloria M. Shenykman, Sricharan Bandhakavi, Lloyd M. Smith, Timothy J. Griffin. 2014. Journal of Proteome Research. 13(12): 5898-908.

sapFinder: an R/Bioconductor package for detection of variant peptides in shotgun proteomics experiments

Bo Wen, Shaohang Xu, Gloria M. Sheynkman, Qiang Feng, Liang Lin, Quanhui Wang, Xun Xu, Jun Wang, Siqi Liu. 2014. Bioinformatics (Oxford, England). 30(21): 3136-8.

Wen et al. 2014 Fig 1

Sheynkman et al. 2014 Fig 1

Using Galaxy-P to leverage RNA-Seq for the discovery of novel protein variations

Gloria M Sheynkman, James E Johnson, Pratik D Jagtap, Michael R Shortreed, Getiria Onsongo, Brian L Frey, Timothy J Griffin, & Lloyd M Smith.  2014. BMC Genomics. 15: 703.

Discovery and mass spectrometric analysis of novel splice-junction peptides using RNA-Seq

Gloria M. Sheynkman, Michael R. Shortreed, Brian L. Frey, Lloyd M. Smith . 2013. Molecular & Cellular Proteomics. 12(8): 2341-53.

Splice junction peptides

Sheynkman et al. 2013 Abstract

Large-scale mass spectrometric detection of variant peptides resulting from nonsynonymous nucleotide differences

Gloria M. Sheynkman, Michael R. Shortreed, Brian L. Frey, Mark Scalf, Lloyd M. Smith. 2013. Journal of Proteome Research. 13(1): 228-40.

Absolute quantification of prion protein (90-231) using stable isotope-labeled chymotryptic peptide standards in a LC-MRM AQUA workflow

Robert Sturm, Gloria Sheynkman, Clarissa Booth, Lloyd M. Smith, Joel A. Pedersen, & Lingjun Li. 2012. Journal of the American Society for Mass Spectrometry. 23(9): 1522-33.

Sturm et al. 2012 Figure 5

Wu et al. 2011 Figure 1

Sequence-specific capture of protein-DNA complexes for mass spectrometric protein identification

Cheng-Hsien Wu, Siyuan Chen, Michael R. Shortreed, Gloria M. Kreitinger, Yuan Yuan, Brian L. Frey, Yi Zhang, Shama Mirza, Lisa A. Cirillo, Michael Olivier, Lloyd M. Smith. 2011. PloS One. 6(10): e26217.

Alkylating tryptic peptides to enhance electrospray ionization mass spectrometry analysis

Suzanne E. Kulevich, Brian L. Frey, Gloria Kreitinger, Lloyd M. Smith. 2010. Analytical Chemistry. 82(24): 10135-42.

Kulevich et al. 2010 graphical abstract

Lee et al. 2007 Figure 5

Metabolism of a highly selective gelatinase inhibitor generates active metabolite

Mijoon Lee, Adriel Villegas-Estrada, Giuseppe Celenza, Bill Boggess, Marta Toth, Gloria Kreitinger, Christopher Forbes, Rafael Fridman, Shahriar Mobashery, Mayland Chang. 2007. Chemical Biology & Drug Design. 70(5): 371-82.