High throughput RNA sequencing technologies

 

 

 

 

 

 

The most prominent sequencing technologies are Illumina and PacBio

Illumina provides next generation sequencing (NGS), which is a fast, scalable, ultra-high throughput massively parallel sequencing technology. It is used to determine nucleotide order for genomes, targeted DNA regions, or RNA.

 

PacBio offers a sequencing technology we commonly use in the Sheynkman Lab called Kinnex for scalable, cost-effective RNA sequencing with increased throughput. Kinnex is long read sequencing for full-length RNA, single-cell RNA, and 16S rRNA that is based on the MAS-Seq method concatenating small amplicons into large fragment libraries.

 

Oxford Nanopore offers DNA and RNA sequencing platforms that use nanopore-based technology to read long strands of genetic material in real time. Unlike traditional sequencing methods, Oxford Nanopore’s devices—ranging from portable units like the MinION to high-throughput platforms like the PromethION—allow for flexible, scalable, and rapid analysis of genomes, transcriptomes, and epigenomes. Their technology is widely used in research, clinical, environmental, and even field-based applications around the world.