SDS Polyacrylamide Gels

Resolving Gels (30:0.14 ratio Acrylamide-Bis low bis)

all volumes in mls  (Note: Two Gel is 1.5X the amounts)

Pour. Overlay with water-saturated n-butanol (2-methyl propanol). The gel should polymerize in about 30 minutes. Pour off alcohol, rinse 2X with distilled water . Dry top of gel thoroughly with filter paper inserted to the level of the gel.

TypeOne Gel 12%One Gel 15%One Gel 18%Two Gels 12%Two Gels 15%Two Gels 18%
1.5 M Tris, pH 8.6101010151515
40% Acrylamide solution1215181822.527
2% Bis solution1.121.41.681.682.12.52
10% SDS.
1% APS222333

Stacking Gels (5% Acrylamide 0.13% Bis)

Pour stacking gel between the glass plates to the very top. Insert comb, allowing it to displace some of the liquid. (Remember acrylamide is a neurotoxin). This should avoid bubbles forming. Let sit about 30 minutes or until polymerized.

TypeOne GelTwo Gel
1M Tris- HCl pH
40% Acrylamide Solution1.8752.8125
2% Bis Solution.9751.4625
10% SDS.15.225
1% APS.751.125