Rates July 2017
Next Gen Sequencing
| Service | University of Virginia | outside rate |
|---|---|---|
| MiSeq Run Setup | $231.00 | $371.91 |
| MiSeq kit, 50 cycles (requires run setup) | $810.00 | $810.00 |
| MiSeq kit, 150 cycles (requires run setup) | $885.00 | $885.00 |
| MiSeq kit, 300 cycles (requires run setup) | $1025.00 | $1025.00 |
| MiSeq kit, 500 cycles (requires run setup) | $1,150.00 | $1,150.00 |
| MiSeq kit, 600 cycles (requires run setup) | $1485.00 | $1485.00 |
| NextSeq Run setup (select a kit below) | $231.00 | $371.91 |
| NextSeq kit – 75 cycle High Output Kit (requires Run setup) | $1,410.00 | $1,410.00 |
| NextSeq kit – 150 cycle High Output Kit (requires Run setup) | $2,675.00 | $2,675.00 |
| NextSeq kit – 150 cycle Mid Output Kit (requires Run setup) | $1,040.00 | $1,040.00 |
| NextSeq kit – 300 cycle High Output Kit (requires Run setup) | $4,260.00 | $4,260.00 |
| NextSeq kit – 300 cycle Mid Output Kit (requires Run setup) | $1,650.00 | $1,650.00 |
| Next Gen Sequencing data analysis, per hour | $85 | $136.85 |
Next Generation Library Preparation
| Service | University of Virginia | outside rate |
|---|---|---|
| Pippin Prep, core run per sample (4 or more samples) | $22.00 | $35.42 |
| Pippin Prep, user run per cartridge (user provided consumables) | $30 | $48.30 |
| Covaris use for DNA shearing – 1st sample, user operated | $45.00 | $72.45 |
| Covaris use, each additional sample, user operated |
$20.00 | $32.20 |
| NGS library prep RNA-seq M using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® | $262 | $421.82 |
| NGS library prep RNA-seq R using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® and NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) | $360 | $579.60 |
| NGS library prep-16s bacteria | $50 | $80.5 |
Quality control and quantitation
| Service | University of Virginia | outside rate |
|---|---|---|
| Quantitation by absorbance with NanoView | $2.00 | $3.22 |
| Quantitation by fluorescence with QuBit | $3.00 | $4.83 |
| Agilent Tape Station using RNA kit | $12.00 | $19.32 |
| Agilent Tape Station using RNA HS kit | $13.00 | $20.93 |
| Agilent Bioanalyzer using DNA HS kit | $13.00 | $20.93 |
| Agilent Bioanalyzer, user run | $25.00 |
PCR
| Service | University of Virginia | outside rate |
|---|---|---|
| qPCR-QuantStudio 6 Flex plate run |
$85 | $136.85 |
| qPCR-QC for NGS library |
$12 | $19.32 |
| ddPCR -user operated – first 8 samples | $45 | $72.45 |
| ddPCR – user operated – additional 8 samples | $25 | $40.25 |
| ddPCR-analysis by core-first 8 samples |
$65 | $104.65 |
| ddPCR analysis by core – additional 8 samples | $45 | $72.45 |