Training Offered
Training Offered
We provide training protocols and assistance. For more information about the training offered at the Pediatric Center of Excellence in Nephrology, please contact Dr. Sequeira Lopez or Dr. Medrano.
- Acute central blood pressure measurement.
- Ambulatory radio telemetry blood pressure, temperature and ECG recordings (with DSI units).
- Partial and total unilateral ureteral obstruction and release in neonatal and adult mice.
- Complete unilateral ureteral obstruction in adult mice.
- Transplantation of embryonic organs under the kidney capsule.
- Nephrectomy.
- Kidney ischemia-reperfusion injury.
- Bone marrow transplantation.
- In vitro colony forming assays of hematopoietic cells.
- Tissue harvesting
- Tissue embedding
- Paraffin and Frozen sections
- Histochemical stainings (Hematoxylin and eosin, PAS, Mason’s Trichrome, Sirius Red, etc..)
- Immunostaining
- In situ hybridization
- Clearing techniques
- Microdissection
- Analysis and quantification using Metamorph
- Cloning
- CRISPR technology
- Targeting design
- Single Cell Genomics