Equipment
Microscopes
Zeiss Lightsheet 7
Standalone sealed box system (laser safe) for multiview imaging of living and optically cleared specimens up to 2cm in size at subcellular resolution given any refractive index between 1.33 – 1.58- Laser lines: 405nm, 445nm, 488nm, 515nm, 561nm, 638nm
- Objectives: 5x, 20x
- Live cell imaging: includes sample chamber providing heating & cooling
- Inverted spectral imaging confocal microscope
- 40 nm xy-spatial resolution for fixed specimen
- tauSTED Depletion lines : 660 nm, and 775 nm
- White Light Laser lines : 440-790 nm
- FALCON (FAst Lifetime CONtrast) imaging
Zeiss LSM 880 Confocal w/ AiryScan
Inverted spectral imaging confocal microscope equipped with AiryScan/fastAiryScan which enables 1.7x higher resolution images at high speed acquisition- Equipped with a 34 channel spectral array
- Laser lines: 405nm, 458nm, 488nm, 514nm, 543nm, 594nm and 633nm
- Objectives: 10x, 20x, 40x, and 63x
- Long-term live cell imaging: includes a fully enclosed CO2 chamber
LSM 880 Confocal – Operation Instructions
Zeiss LSM 700 Confocal
Inverted microscope ideal for collecting transmitted light images as well as conventional confocal fluorescence images- Equipped with 4 channel detection
- Laser lines: 405, 488, 543, 633 nm
- Objectives: 10x, 20x, 40x, and 63x
- Short-term live cell imaging: includes a CO2 incubator
Leica STELLARIS 8 DIVE Multiphoton Confocal
- Inverted spectral imaging confocal microscope
- a tunable Coherent Chameleon Vision-S laser for Multiphoton (2-photon) imaging in 1mm depth or beyond
- 4Tune HyD NDD 2 channel
- 3x Power HyD S
- 1x Power HyD X
- White Light Laser
- LIGHTNING
Zeiss LSM 710 Multiphoton Confocal
Upright laser scanning confocal microscope with a tunable Coherent Chameleon Vision-S laser for Multiphoton (2-photon) imaging which allows deeper imaging of tissues- Equipped with 4 channel detection
- Laser lines: 405nm, 458nm, 488nm, 514nm, 561nm, and 633nm
- Objectives: 10x, 20x, 63x
Leica Thunder Imager 3D Cell Culture w/ TIRF
Fully automated inverted microscope equipped with a W-View Gemini for dual color TIRF as well as color and monochromatic cameras for widefield and brightfield, respectively- Laser lines for TIRF: 405, 488, 561, and 638nm
- Objectives: 5x, 10x, 20x, 40x, 63x
- Live cell imaging: includes CO2 connection with stage top incubation
Zeiss Sigma VP HD SEM
The Zeiss SIGMA VP-FESEM is a variable pressure field emission scanning electron microscope, which allows users to perform high-resolution SEM work for conventional secondary electron imaging, backscattered electron imaging, and 3View reconstruction.
Zeiss Sigma Scanning Electron Microscope – Operation Instructions
Acquifer HIVE
- Intel CPU 32 Processing Cores (2×16 core @ 2.8GHz)
- 1.5T of RAM
- NVIDIA Quadro RTX A6000 (48GB) GPU
- 10 TB SSD fast and secure local storage volume
- RAID6 78TB upgradeable file server
- 10Gbit local network
WorkStation 1
- Intel(R) Xeon(R) W-2135 CPU @ 3.70GHz
- 256GB RAM
- NVIDIA Quadro P2000
- 64-bit operating system
- 500GB SSD and 2TB HDD
Workstation 2
- Intel(R) Xeon(R) CPU E5-2620V3 CPU @ 2.4 GHz (2 processors)
- 32 GB RAM
- NVIDIA Geforce GTX1080
- 64-bit operating system
- 2TB SSD and 2TB HDD
Analysis Software
The Core has licenses and service agreements for cutting-edge image analysis software.
- 4D Analysis
- 2D&3D Analysis
- Zen Blue
- Leica Aivia
- LAS X
- Deconvolution
- ClearView
- Open Source
Lightsheet Microscopy Sample Preparation
LifeCanvas SmartBatch+ Tissue Clearing and Immunolabeling- Active 3D tissue clearing (Clear+ method) and immunolabeling (eFLASH with stochastic electrotransport technologies) for intact tissue volumes
- Duration: ~2 weeks w/ immunolabeling (endogenous FPs absent) or ~1 week w/o immunolabeling (endogenous FPs present)
Full Active Pipeline Protocol SmartBatch+ v5.05
Electron Microscopy Sample Preparation
- Leica Ultracut Ultramicrotomes (3)
- Critical Point Dryer
- Sputter Coater
- Olympus BX51 microscope/digital camera: Brightfield, Fluorescence, Phase Contrast and DIC
- Olympus SZX12 dual objective stereo microscope/digital camera: Brightfield and Darkfield