Leica TIRF System Specs
| Microscope Stand | Leica DMi8 |
| Illumination | Leica Multi-Line LED 390/479/555/635 DFT and QSPC-T 440/510/575/747 CYR |
| Dichroics and Emission | Filter cube QSP-T Excitation: 375/70, 488/20, 561/20, 638/20 Dichroic: 417, 506, 576, 655 Emission 444/48, 524/32, 600/36, 731/144. Filter set DFT51011 with Emission filter wheel Excitation: 391/32, 479/33, 554/24, 638/31 Dichroic: 415, 500, 572, 660 Emission: 435/30, 519/25, 594/32, 695/58 External 1: 440/40 2: 510/40 3: 590/50 4: 700/75 Filter cube CYR71010 Excitation: 436/28, 506/21, 578/24, 730/40 Dichroic: 459, 523, 598, 763 Emission: 473/22, 539/24, 641/78, 810/80 |
| Cameras | Leica DFC9000 GTC monochromatic Leica DMC4500 color |
| Objectives | N PLAN 5x/0.12 PH0 HC PL FLUOTAR 10x/0.32 PH1 HC PL FL L 20x/0.40 CORR PH1 HC PL APO 20x/0.80 HC PL APO 40x/0.95 CORR HC PL APO 63x/1.40-0.60 OIL HC PL APO 100x/1.47 OIL CORR TIRF |
| Incubator | Tokai STXG-DMIWX-SET |
| XY-stage | Motorized X-Y and piezo-Z |
| Software | LAS X |
| HP TIRF lasers | 405nm 50mW 488nm 150mW 561nm 120mW 638nm 150mW |