Digital PCR

The Genome Analysis and Technology Core is currently establishing new digital PCR services supported by the Qiagen QIAcuity instrument. This instrument is offered as a shared instrument operated by trained users at this time.

Please contact Alyson Prorock to set up a training time. Please also view this instructional video provided by QIAgen:

The QIAcuity Digital PCR System is a nanoplate-based system which integrates a standard dPCR workflow of partitioning, thermocycling and imaging into a walk-away automated platform with minimal hands-on time.

Often times the user is able to adapt current qPCR assays to the QIAcuity Digital PCR System for quick results in under 2 hours.

The QIAcuity Digital PCR System will deliver precise and multiplexed quantification results for all molecular applications including but not limited to the following areas:

• Rare mutation detection
• Copy number variation analysis
• Gene expression analysis
• NGS library quantification
• Pathogen detection
• Genotyping
• miRNA research