Next Generation Sequencing (NGS)

NGS technology utilizes a massively parallel approach to quantitatively assess copy number and sequence changes in DNA or RNA samples. It has become the preferred method for integrated approaches that simultaneously measure transcript copy numbers and sequence-based alterations. Two NGS instruments are currently available in the core: Illumina NextSeq™2000 and Illumina MiSeq Sequencing Systems. The NSQ2K is the newest addition and has replaced the formerly available NSQ500.

Each instrument supports sequencing kits that offer a range of possible cycle numbers and read output. The NSQ2K P3 flow cell offers a max output of 330 Gb or 1.2 Billion reads per run, and supports varying read lengths of 50, 100, 200 and 300 bp.  The P2 flow cell offers a max output of 120 Gb or 400 Million reads per run, and supports read lengths of 100, 200 or 300 bp.

Please keep this in mind as you are making your kit selection in iLab. Our staff can assist you in selecting the appropriate sequencing kit for your study.

Applications

  • Whole-genome resequencing
  • Targeted resequencing, including exome panels and custom enrichment panels (100s kb–Mbs)
  • Whole-genome de novo sequencing
  • Sequencing of bisulfite-treated DNA (Methyl-Seq)
  • RNA sequencing (RNA-Seq), including mRNA sequencing and total RNA sequencing (coding and noncoding)
  • Genotyping by sequencing
  • Nucleosome positioning and chromatin structure studies
  • ChIP-Seq: studying sequence-specific protein–RNA interactions
  • Sequencing of ancient DNA samples
  • Single-cell applications including RNA-Seq, VDJ, Cell surface expression, ATAC-seq
  • Linked long-read applications

To discuss projects, contact Katia Sol-Church at ks5uq@virginia.edu ph. 434.924.9953 or Yongde Bao yb8d@virginia.edu ph. 434.924.2553.