Sample Requirements

To help ensure you get the quality of data you expect, please follow the sample requirements below.

 

Service Required sample volume Sample concentration Sample container Comments
NanoVue – RNA or DNA 2.5 µL 5 ng/µL or higher 1.5 ml tube
Qubit – DNA HS 2 µL 0.2 – 100 ng/µL 1.5 ml tube
Qubit – RNA HS 2 µL 5 – 100 ng/µL 1.5 ml tube
Agilent – Tape Station – RNA kit 2 µL 25 – 500 ng/μL 8 tube strip or 96 well plate
Agilent – Tape Station – RNA HS kit 3 µL 0.5 – 10 ng/μL 8 tube strip or 96 well plate
Agilent – Tape Station – DNA5000 HS kit 3 µL 100 – 5000 bp, 10 – 1000 pg/μL 8 tube strip or 96 well plate
Agilent – Bioanalyzer – DNA HS 2 µL 50 – 7000 bp, 5 – 500 pg/μL 1.5 ml tube
Pippin Prep – core run per sample up to 30 µL 5 ng – 10 µg of material 1.5 ml tube
NGS library prep – 10X Genomics – single cell RNAseq 1 ml 500 cells/ µL in PBS + 0.04% non-acetylated BSA Wash and resuspend in 1.5ml low binding tube Use wide bore low binding pipet tips, see core for specifics
NGS library prep – RNAseq – mRNA isolation up to 50 µL 100 ng – 1 µg in up to 50 µL, 1 µg preferred (20ng/µL) 8 tube strip or 96 well plate Additional material for NanoVue and Agilent REQUIRED
NGS library prep – RNAseq – rRNA depletion up to 12 µL 100 ng – 1 µg in up to 12 µL, 1 µg preferred (83 ng/µL) 8 tube strip or 96 well plate Additional material for NanoVue and Agilent REQUIRED
NGS Illumina NextSeq Run Setup 18.2 µL – 5 µL 1.1 nM – 4 nM 1.5 ml tube This does not leave any excess for QC – additional volume for Qubit, Agilent & qPCR recommended
NGS Illumina MiSeq Run Setup 18.2 µL – 5 µL 1.1 nM – 4 nM 1.5 ml tube This does not leave any excess for QC – additional volume for Qubit, Agilent & qPCR recommended