Sample Requirements

To help ensure you get the quality of data you expect, please follow the sample submission requirements below. Please contact us with questions or if you still want services performed but have samples that do not meet the minimum requirements.

 

Service
Required sample volumeSample concentrationSample container
Comments
NanoVue – RNA or DNA2.5 µL
5 ng/µL or higher
1.5 ml tube
Qubit – DNA HS
2 µL
0.2 – 100 ng/µL
1.5 ml tube
Qubit – RNA HS
2 µL
5 – 100 ng/µL
1.5 ml tube
Agilent – Tape Station – RNA kit2 µL
25 – 500 ng/μL
8 tube strip or 96 well plate
Agilent – Tape Station – RNA HS kit3 µL
0.5 – 10 ng/μL
8 tube strip or 96 well plate
Agilent – Tape Station – DNA5000 HS kit
3 µL
100 – 5000 bp, 10 – 1000 pg/μL8 tube strip or 96 well plate
Agilent – Bioanalyzer – DNA HS2 µL
50 – 7000 bp, 5 – 500 pg/μL1.5 ml tube
NGS library prep – 10X Genomics – single cell RNAseq1 ml
500 cells/ µL in PBS + 0.04% non-acetylated BSAWash and resuspend in 1.5ml low binding tubeUse wide bore low binding pipet tips, see core for specifics
NGS library prep – RNAseq – mRNA isolationup to 50 µL
10 ng – 1 µg in up to 50 µL, 1 µg preferred (20ng/µL)8 tube strip or 96 well plateAdditional material for NanoVue and Agilent REQUIRED
NGS library prep – RNAseq – rRNA depletionup to 12 µL
10 ng – 1 µg in up to 12 µL, 1 µg preferred (83 ng/µL)8 tube strip or 96 well plate
Additional material for NanoVue and Agilent REQUIRED
NGS Illumina NextSeq Run Setup18.2 µL – 5 µL
1.1 nM – 4 nM minimum
1.5 ml tube
This does not leave any excess for QC – additional volume for Qubit, Agilent & qPCR recommended
NGS Illumina MiSeq Run Setup18.2 µL – 5 µL
1.1 nM – 4 nM minimum
1.5 ml tube
This does not leave any excess for QC – additional volume for Qubit, Agilent & qPCR recommended