| NanoVue – RNA or DNA | 2.5 µL
| 5 ng/µL or higher
| 1.5 ml tube
| |
Qubit – DNA HS
| 2 µL
| 0.2 – 100 ng/µL
| 1.5 ml tube
| |
Qubit – RNA HS
| 2 µL
| 5 – 100 ng/µL
| 1.5 ml tube
| |
| Agilent – Tape Station – RNA kit | 2 µL
| 25 – 500 ng/μL
| 8 tube strip or 96 well plate
| |
| Agilent – Tape Station – RNA HS kit | 3 µL
| 0.5 – 10 ng/μL
| 8 tube strip or 96 well plate | |
Agilent – Tape Station – DNA5000 HS kit
| 3 µL
| 100 – 5000 bp, 10 – 1000 pg/μL | 8 tube strip or 96 well plate | |
| Agilent – Bioanalyzer – DNA HS | 2 µL
| 50 – 7000 bp, 5 – 500 pg/μL | 1.5 ml tube
| |
| NGS library prep – 10X Genomics – single cell RNAseq | 1 ml
| 500 cells/ µL in PBS + 0.04% non-acetylated BSA | Wash and resuspend in 1.5ml low binding tube | Use wide bore low binding pipet tips, see core for specifics |
| NGS library prep – RNAseq – mRNA isolation | up to 50 µL
| 10 ng – 1 µg in up to 50 µL, 1 µg preferred (20ng/µL) | 8 tube strip or 96 well plate | Additional material for NanoVue and Agilent REQUIRED |
| NGS library prep – RNAseq – rRNA depletion | up to 12 µL
| 10 ng – 1 µg in up to 12 µL, 1 µg preferred (83 ng/µL) | 8 tube strip or 96 well plate
| Additional material for NanoVue and Agilent REQUIRED |
| NGS Illumina NextSeq Run Setup | 18.2 µL – 5 µL
| 1.1 nM – 4 nM minimum
| 1.5 ml tube
| This does not leave any excess for QC – additional volume for Qubit, Agilent & qPCR recommended |
| NGS Illumina MiSeq Run Setup | 18.2 µL – 5 µL
| 1.1 nM – 4 nM minimum
| 1.5 ml tube
| This does not leave any excess for QC – additional volume for Qubit, Agilent & qPCR recommended |
