Title: Designing, and evolving, genetically encoded biosensors to visualize cell physiology

Speaker: Dr. Robert Campbell

Professor of Science, Chemistry
University of Alberta, Canada and University of Tokyo, Japan

 

Host: Dr. Huiwang Ai

Abstract: The Campbell research group is focused on the use of protein engineering for the development of fluorescent protein-based biosensors for imaging of cell signalling and metabolism. Protein engineering, using a combination of rational protein design and directed protein evolution, is the most effective and versatile approach for generating new genetically encoded fluorescent biosensors. By exploiting structure-guided design, iterative cycles of high-throughput fluorescence image-based screening of bacterial colonies, and lower throughput testing of promising variants in mammalian cells, we are developing a growing selection of fluorescent protein-based biosensors with improved properties. In this seminar I will present some of our most recent efforts to engineer an improved generation of biosensors. Specifically, I will provide an update on the expanding palette of calcium ion biosensors, and describe how we are using similar engineering efforts to make biosensors for neurotransmitters, ions, and key metabolites.

Date: Monday May 4, 2020
Time: 4:00 PM – 5:00 PM

Location: Pinn Hall, 3016 Cell Bio Large Conference Room

For Information Contact: Jennifer Nickerson Email Contact Person