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Current Bielinsky-Hendrickson Publications

Contribution of HMGB1 to Keratinocyte inflammation in Recessive Dystrophic Epidermolysis Bullosa

bioRxiv [Preprint]. 2025 Aug 11:2025.08.11.668806. doi: 10.1101/2025.08.11.668806.ABSTRACTRecessive dystrophic epidermolysis bullosa (RDEB) is an inherited skin disorder characterized by fragile skin, blistering, and chronic wounds. Keratinocytes, the primary cells in the…

The Ski2 helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses

bioRxiv [Preprint]. 2025 Jul 26:2025.07.24.666583. doi: 10.1101/2025.07.24.666583.ABSTRACTThe activating signal co-integrator 1 complex subunit 3 (ASCC3), the largest subunit of ASCC, is one of two Ski2-like helicases with duplicated helicase cassettes…

Gene editing and CRISPR-dependent homology-mediated end joining

Exp Mol Med. 2025 Jul;57(7):1409-1418. doi: 10.1038/s12276-025-01442-z. Epub 2025 Jul 31.ABSTRACTGene editing is the intentional modification of a genetic locus in a living cell and is used for two general…

Cell Type Specific Suppression of Hyper-Recombination by Human RAD18 Is Linked to Proliferating Cell Nuclear Antigen K164 Ubiquitination

Biomolecules. 2025 Jan 20;15(1):150. doi: 10.3390/biom15010150.ABSTRACTRAD18 is a conserved eukaryotic E3 ubiquitin ligase that promotes genome stability through multiple pathways. One of these is gap-filling DNA synthesis at active replication…

Cell type specific suppression of hyper-recombination by human RAD18 is linked to PCNA K164 ubiquitination

bioRxiv [Preprint]. 2024 Sep 3:2024.09.03.611050. doi: 10.1101/2024.09.03.611050.ABSTRACTHomologous recombination (HR) and translesion synthesis (TLS) promote gap-filling DNA synthesis to complete genome replication. One factor involved in both pathways is RAD18, an…

Improved detection of DNA replication fork-associated proteins

Cell Rep. 2024 May 28;43(5):114178. doi: 10.1016/j.celrep.2024.114178. Epub 2024 May 2.ABSTRACTInnovative methods to retrieve proteins associated with actively replicating DNA have provided a glimpse into the molecular dynamics of replication…

RNF4 prevents genomic instability caused by chronic DNA under-replication

DNA Repair (Amst). 2024 Mar;135:103646. doi: 10.1016/j.dnarep.2024.103646. Epub 2024 Feb 7.ABSTRACTEukaryotic genome stability is maintained by a complex and diverse set of molecular processes. One class of enzymes that promotes…

A scalable platform for efficient CRISPR-Cas9 chemical-genetic screens of DNA damage-inducing compounds

Sci Rep. 2024 Jan 30;14(1):2508. doi: 10.1038/s41598-024-51735-y.ABSTRACTCurrent approaches to define chemical-genetic interactions (CGIs) in human cell lines are resource-intensive. We designed a scalable chemical-genetic screening platform by generating a DNA…

A critical threshold of MCM10 is required to maintain genome stability during differentiation of induced pluripotent stem cells into natural killer cells

Open Biol. 2024 Jan;14(1):230407. doi: 10.1098/rsob.230407. Epub 2024 Jan 24.ABSTRACTNatural killer (NK) cell deficiency (NKD) is a rare disease in which NK cell function is reduced, leaving affected individuals susceptible…

FANCD2-dependent mitotic DNA synthesis relies on PCNA K164 ubiquitination

Cell Rep. 2023 Dec 26;42(12):113523. doi: 10.1016/j.celrep.2023.113523. Epub 2023 Dec 5.ABSTRACTUbiquitination of proliferating cell nuclear antigen (PCNA) at lysine 164 (K164) activates DNA damage tolerance pathways. Currently, we lack a…

RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication

Open Biol. 2023 Aug;13(8):230068. doi: 10.1098/rsob.230068. Epub 2023 Aug 23.ABSTRACTDNA replication requires precise regulation achieved through post-translational modifications, including ubiquitination and SUMOylation. These modifications are linked by the SUMO-targeted E3…

Fanconi anemia-associated chromosomal radial formation is dependent on POLθ-mediated alternative end joining

Cell Rep. 2023 May 30;42(5):112428. doi: 10.1016/j.celrep.2023.112428. Epub 2023 Apr 21.ABSTRACTActivation of the Fanconi anemia (FA) pathway after treatment with mitomycin C (MMC) is essential for preventing chromosome translocations termed…

Publications (10 years) Prior to 2023