Mass Spectrometry

Request mass spectrometry services through iLab, see Instructions

Analyses available:

• Protein identification and sequencing from gel or solution by ESI-LC/MS/MS at the low femtomole level (Simple Mixture MS/MS service).
• Analysis of protein mixtures such as tissue, media, and whole cells (Complex Mixture MS/MS service)
• Proteomic Quantification, comparison of proteins in samples using isotopic labels (TMT – Tandem Mass Tags) or label free analysis
• Absolute quantitation of proteins using labeled peptides and parallel reaction monitoring (PRM service)
• Identification of phosphorylation sites (titanium dioxide enrichment if complex)
• Identification of post-translational modifications (e.g.  ubiquitination, acetylation, methylation, etc.) (PTM service)
• High resolution, high mass accuracy measurements of peptides and small proteins by CE-ESI-MS
• Mass determination by MALDI mass measurements for confirming identity of peptides and proteins
• Qualitative/Quantitative analysis of metabolites, drugs and small molecules

Good sample preparation is vital in getting good data. When starting a new project, read our guidelines and discuss the project with Dr. Sherman or Dr. Silva.