Reagents For Use with forteBIO Molecular Interactions Instrument
Required and suggested supplies for fortéBIO Octet RED96
These validated plates must be used:
96 well, black, flat bottom, polypropylene, 200 µL well.
Greiner Cat. No. 655209. VWR Cat. No. 82050-784. Fisher Cat. No. 50-320-828
There are multiple Octet models, some of which can use other plates. We have the Octet RED96 for which only the plates above can be used.
Kits suggested by fortéBIO.
EZ-Link NHS-PEG4-Biotin (Thermo, part no. 21329)
NHS-PEG12-Biotin (Thermo, part no. 21312)
Sulfo-NHS-LC-LC-Biotin (Thermo, part no. 21338)
Suggested by fortéBIO
Zeba Spin Desalting Columns, 0.5mL, 25 columns (Thermo, part no. 89882)
Zeba Spin Desalting Columns, 0.5mL, 50 columns (Thermo, part no. 89883)
For desalting 30-130 µL of compounds larger than 7,000 Da
EZ-Link biocytin, 100 mg. Thermo part 28022
Buffers
Kinetics buffer, 50 ml of 10X, fortéBIO part 18-5032
reagent/sample diluent (based on PBS), fortéBIO part 18-5028, for quantitation sensors