Background on Molecular Interactions Using BLI

Some questions about BioLayer Interferometry and its application are answered below, and many more on the sartorius web site. To see some literature on the Sartorius website, you need to register as a user with Sartorius.


Probe life

Amount of material bound on a probe

Sample volume in a well of a 96 well plate

Association rates that can be measured

Dissociation rates that can be measured

Dissociation constant range

Molecular weight range for analytes

Protein quantitation range for hIgG

6-12 months

1.6 fmole

180-220 µL

ka 101 to 107 M-1 s-1

kd 10-6 to 10-1 s-1

10-3 to 10-11M

150 Da to 200 nm diamter

0.05 to 100 µg/mL (lower limits for some assays)