Biospecimen procurement, processing & banking

  • Human tissue procurement from clinical specimens and preservation
    • Surgical resection specimens (targeted collection of remnant material)
    • Autopsy specimens (targeted collection of remnant material)
    • Study-specific collection of non-remnant material (consented)
  • Banked specimens for future research (>30,000 specimens available)
  • Biofluid sample processing
    • Clinical trial and biobanking support for UVA investigators-initiated studies
  • Enzymatic dissociation & viable freezing of tissue samples
  • Peripheral blood leukocyte (PBL) Ficoll isolation & viable freezing
  • Extraction of nucleic acids and protein from tissue samples  including microcapillary electrophoresis and RNA amplification & labeling

Histology-based research techniques

For basic histology services (processing/embedding/sectioning) involving animal tissues, please use the Research Histology Core Facility. The BTRF does accept animal tissues for IHC, CISH, and other advanced applications.
  • Paraffin embedding of human tissue specimens
  • Histologic sectioning of fixed human tissue specimens
  • Cryostat sectioning of frozen human tissue specimens
    • Training on instrument
    • Equipment usage
  • Cytospin preparation of fluids containing cells
  • Histology-guided macrodissection to enrich for specific cell types
  • Immunohistochemistry (IHC)
    • Performed on human and animal tissues
    • Panel of “standard” antibodies (for more information click here)
    • Custom immunohistochemistry (Investigator-supplied antibody)
  • Chromogenic RNA In Situ Hybridization (CISH)
  • GeoMx® DSP services
  • Custom tissue microarray manufacture
  • Tissue microarray of the most common human carcinomas (159 cases)