Chromogenic RNA in situ hybridization


The BTRF performs chromogenic RNA in situ hybridization (CISH) on histologic tissue sections. This powerful technique allows the specific localization of RNA targets in fixed tissues and cells, thus providing correlation of specific gene expression with tissue compartments and cellular morphology. Efficient single molecule visualization in individual cells is achieved through the use of a novel probe design strategy and a hybridization-based signal amplification system (branched DNA signal amplification) to simultaneously amplify signals and suppress background. Probe sets can be designed to any RNA of choice. Signal can be detected using standard bright field microscopy. The assays offer choice of two chromogens, brown (DAB) and red (Fast Red) on formalin-fixed paraffin embedded (FFPE) or frozen histologic sections. At present, we offer RNA ISH assays utilizing  Invitrogen (ThermoFisher) QuantiGene™ ViewRNAⓇ  and Advanced Cell Diagnostics RNAscope™ products. This assay provides the ability to analyze gene expression in situ  in clinical and research tissue specimens.

Please feel free to schedule an appointment with the Director of the BTRF for further information or to discuss your specific requirements.