Immunofluorescence (IF) Staining

Immunofluorescence (IF) a powerful tool enabling the visualization and colocalization of many proteins and biomolecules within a single tissue with an accompanying array of fluorophores that cover the entire visual spectrum.

We provide high-quality and efficient services for full assay development, protocol validation, single/multiplex staining, image collection, and data analysis. The BTRF performs IF on FFPE and frozen tissue, tissue microarrays (TMAs) and on cytology preparations for human and mouse tissues in both direct and indirect methods of fluorescence staining. More importantly, almost all bright-field IHC staining protocols offered at the BTRF can be converted to IF staining. Currently the BTRF provides multiplexing IF up to 5 fluorophores (Dapi, FITC, mCherry/Texas red, CY5 and CY7).

The BTRF provides all secondary and detection reagents.  A “work up” charge is applied for the titration of all new antibodies. The investigator should also determine a positive control tissue. The BTRF can obtain specific human tissues to be used as controls.

Please feel free to schedule an appointment with the Director of the BTRF to discuss your specific requirements

To download an IF order form, click here.