Core Service Database
| Service | Description | Core |
|---|---|---|
| Training on operating microscopes | The core provides training in usage of the microscopes. Training sessions can be scheduled using iLab calendar or arranged in consultation with the facility staff. Once trained, users gain access to the facility to carry out their own image acquisition. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Consultation on microscopy projects | The facility staff offers guidance and expertise in experimental design and approach during consultations. They ensure researchers receive comprehensive support to optimize their experimental methodologies. Facility offers help chosing the microscope which will meet user's needs and expectations. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Lightsheet Microscopy | Lightsheet microscopy is used for imaging of large, three-dimensional biological samples with minimal phototoxicity. Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only fluorophores within the focal volume. LSM have a true optical sectioning capability and, hence, provide axial resolution, restrict photobleaching and phototoxicity to a fraction of the sample and use cameras to record tens to thousands of images per second. LSMs are used for in-depth analyses of large, optically cleared samples and long-term three-dimensional (3D) observations. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Multiphoton Microscopy | Allows to achieve deep tissue imaging with high spatial resolution and minimized photodamage. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Confocal Microscopy including FRAP, FRET, FLIM | FRAP is a fluorescence microscopy method for studying the mobility of fluorescently-labeled molecules in living cells. FLIM is a fluorescence imaging technique where the contrast is based on the lifetime of individual fluorophores rather than their emission spectra. FRET microscopy relies on the ability to capture fluorescent signals from the interactions of labeled molecules in single living or fixed cells. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Live Cell Imaging, Time Lapse, Intravital Imaging | Live cell imaging is the study of cellular structure and function in living cells via microscopy. It enables the visualization and quantitation of dynamic cellular processes in real time. Provides the temperature, CO2 and humidity while imaging. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Scanning Electron Microscopy | Scanning electron microscopy, or SEM, produces detailed, magnified images of an object by scanning its surface to create a high resolution image down to the nanometer scale. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Critical point drying and sputter coating | Sample preparation with critical point drying and sputter coating. Those techniques ensure artifact-free specimens for scanning electron microscopy (SEM), enabling high-resolution imaging and accurate analysis of surface structures. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Sample preparation for Lightsheet Microscopy | Tissue clearing technique that delipidates samples with no change in morphology. System used at the facility combines electrophoretic tissue clearing and immunolabeling into one high-throughput device. Batch processing: 4-12 samples. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Sample preparation for Scanning Electron Microscopy | Optimal sample preparation for scanning electron microscopy (SEM) at our facility. Our meticulous procedure include fixation, dehydration, chemical or critical point drying, ensuring good quality specimens for high-resolution imaging of surface structures. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Assisted imaging | AMF images the samples for the user including wide field, confocal, lightsheet, and SEM. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Image data analysis | AMF performs quantitative data analysis, 3D rendering, and generate figure/videos/stats on microscopy data. We use the following commerical and open source software: Python scripting, machine learning, Imaris, Fiji, CellProfiler, etc. | Advanced Microscopy Facility Director: Sijie Hao, PhD pfa2xb@virginia.edu |
| Custom bioinformatics / biostatistical support | Any type of genomic, transcriptomic, epigenomic, and proteomic data analysis that is tailored towards the project's aim. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Proteomics Data Analysis | Identifying proteins and quantifying their abundances followed by differential protein and gene expression and pathway analyses. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Gene expression profiling (Bulk): RNA-seq and microarray | Raw data QC, adaptor contamination removal (if any), reads mapping to whole genome and transcriptome, transcriptome profiling, differential gene expression analysis, and pathway analysis. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Small RNA-seq data analysis | Raw data QC, adaptor contamination removal (if any), reads mapping to small RNA (microRNA, tRNA, etc.), transcriptome profiling, differential gene expression analysis, and pathway analysis. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Allele-specific gene expression analysis | The raw reads are mapped to the genome and transcriptome, and the gene expression profiles are extracted on an allele-specific basis. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Single Cell RNA-seq (scRNA-seq) (expression profiling, cluster generation, cluster annotation, Marker gene identification, pseudotime trajectory analysis (PHATE, Monocle etc.), pathway analysis, etc.) | Performing pre-alignment data quality control, mapping reads to the genome and transcriptome, post-alignment data quality control, removing ambient nucleic acid contamination, conducting standard QC and clustering, identifying marker genes, annotating clusters, conducting pathway analysis, and performing trajectory analysis, among other tasks. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Single Cell ATAC-seq (scATAC-seq) data analysis | Pre-alignment data quality control, mapping reads to the genome and transcriptome, post-alignment data quality control, ambient nucleic acid contamination removal, standard QC and clustering, marker gene identification, cluster annotation, pathway analysis, trajectory analysis, and other related analyses. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Integrated scRNA-seq and scATAC-seq data analysis | Pre-alignment data quality control, mapping reads to the genome and transcriptome, post-alignment data quality control, ambient nucleic acid contamination removal, standard QC and clustering, marker gene identification, cluster annotation, pathway analysis, trajectory analysis, and other related analyses. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Single Cell Spatial genomics data analysis (CosMx (Nanostring/Bruker), CytAssist – Visium V2 and HD (10x Genomics) etc.) | Data quality control, aligning reads to the genome, transcriptome, and image file, and re-aligning the image (if require). All standard analyses performed for single-cell RNA-seq are included. Additionally, cells are mapped back to the image to determine their origin, and neighborhood analysis is also conducted. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| DNA variation (variant calling and annotation (SNP) and GWAS) | aw data quality check, aligning sequencing reads against a reference genome, identifying and annotating variants, calculating allele frequencies (both within the sample and relative to public databases like gnomAD), and predicting deleterious and benign mutations. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Copy number variation (Aneuploidy, insertion/deletion etc.) | Paired-end raw data quality check, aligning sequencing reads against a reference genome, and identifying insertions, deletions, and duplications in the sequenced genome compared to the reference genome. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Genome-wide quantification of DNA double-strand break | Paired-end reads are mapped to the reference genome, and genomic coordinates of double-strand breaks (DSBs) are identified based on the mapping patterns. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| DNA protein binding (ChIP-seq, CHIRP-seq, etc.) | Raw reads are mapped, and peaks are called based on these mapped reads. Blacklisted peaks are removed to identify the true binding sites. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| RNA binding (CLIP-seq, PAR-CLIP, CLASH, etc.) | In this case, raw reads are mapped to genomic and transcriptomic regions. Clusters of mapped reads are identified, mismatch base frequencies are calculated, and a threshold is set to determine true RNA binding sites. For CLASH data analysis, reads are mapped to two non-contiguous regions in the genome. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| ATAC-seq data analysis | Active or open chromatin regions in the genome are identified, transcription factor binding sites are identified. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| CUT&RUN data analysis | Active or open chromatin regions in the genome are identified. This process is similar to ChIP-seq, but does not require an antibody against the specific binding region of interest. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| DNA methylation: sequence-based analysis and array-based analysis | Quantitative identification and profiling of DNA methylation across the entire genome | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Microbiome data analysis (16S rRNA, shotgun metagenomics, etc.) | Reads are cleaned, low-quality reads are trimmed, and then mapped to bacterial/archaeal 16S reference databases. After mapping, microorganisms are quantified. For metagenomics, reads are mapped to a reference dataset, where clade-specific regions are used to create the reference database. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Metabolomics data analysis (Targeted & Untargeted) | Metabolites are identified and quantified, followed by differential abundance analysis. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Pathway and functional analysis | The input for this analysis can be a list of genes or specific chromosomal regions. The analysis is conducted using various reference databases. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Survival analysis (with TCGA sequencing and clinical data) | Survival analysis utilizes gene expression data, mutation information, clinical details, and patient status. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| CRISPR screening experimental design and data analysis | The goal is to identify genes that confer an advantage or disadvantage in a given selection process. In this case, raw reads are quality checked, adaptor contaminations are removed, and the clean reads are mapped to the reference CRISPR libraries. Downstream analysis is performed using program like MAGEK. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Power Analysis (Estimation of sample size) | This is a crucial analysis that should be conducted before setting up the experiment to ensure we have sufficient statistical power to support or refute the hypothesis. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Grant and manuscript support | In this case, we assist with the bioinformatics components of grant writing and manuscript preparation. | Bioinformatics Core Director: Pankaj Kumar, PhD pk7z@virginia.edu |
| Simple Mixture tandem mass spectrometry (Label free semi-quantification spectral counting) | Protein identification from gel, solution, or on-bead by LC-ESI-MS(MS) at the low level. Generally hundreds to thousand proteins (no fractionation) | Biomolecular Analysis Facility Director: Nicholas Sherman, PhD nes3f@virginia.edu |
| Complex Mixture tandem mass spectrometry (Label free semi-quantification spectral counting) | Protein identification from mixtures such as tissue or whole cells where thousands of protein identifications needed (fractionation and multiple mass spectrometry runs) | Biomolecular Analysis Facility Director: Nicholas Sherman, PhD nes3f@virginia.edu |
| TMT (tandem mass tag) Relative Quantification | Proteomic quantification using labels (6,10,16,18-plex) - needs 30-100ug per sample | Biomolecular Analysis Facility Director: Nicholas Sherman, PhD nes3f@virginia.edu |
| PRM (parallel reaction monitoring) [PRM Setup] | Absolute quantitation of proteins, metabolites, lipids, or drugs. Requires method development for specific molecules. | Biomolecular Analysis Facility Director: Nicholas Sherman, PhD nes3f@virginia.edu |
| PTM (post-translational modification) | Identification of post-translational modifications on one or a few proteins (e.g. ubiquitination, acetylation, methylation, phosphorylation, etc.). Client will have to use a kit to enrich if looking for global (i.e. whole cell) PTMs. | Biomolecular Analysis Facility Director: Nicholas Sherman, PhD nes3f@virginia.edu |
| (HRMA) High Resolution, High Mass Accuracy | High resolution, high mass accuracy measurements of peptides, proteins or small molecules molecular weight. Either LC-ESI-MS or CE-ESI-MS. | Biomolecular Analysis Facility Director: Nicholas Sherman, PhD nes3f@virginia.edu |
| Mass Determination by MALDI | Mass measurements for confirming identity of peptides, proteins, and small molecules (lower resolution and mass accuracy) | Biomolecular Analysis Facility Director: Nicholas Sherman, PhD nes3f@virginia.edu |
| Metabolomics / Lipidomics | Identification of metabolites and/or lipids showing those that change (relative quantification) | Biomolecular Analysis Facility Director: Nicholas Sherman, PhD nes3f@virginia.edu |
| Consultation | NMR provides information about molecular structure and dynamics at atomic resolution. Consultation on how to use NMR to achieve your goals is available. | Biomolecular Magnetic Resonance Facility Director: Jeff Ellena, PhD jfe@virginia.edu |
| Project Assistance | Collection and/or analysis of NMR spectra are available. | Biomolecular Magnetic Resonance Facility Director: Jeff Ellena, PhD jfe@virginia.edu |
| Provides human tissues and fluid samples | BTRF provides tissue samples from remnant surgical resection and autopsy specimens linked to clinicopathologic data while maintaining patient confidentiality. FFPE, frozen and fresh tissues are available. BTRF also collect, process and store bodily fluid samples from cancer patients and other diseses. Serum, plasma, saliva, buccal swab, buffy coat, PBMC and wholeblood specimens from cancer and COVID-19 patients are available. This requires appropriate UVA Institutional Review Board for Health Sciences Research (IRB-HSR) approval or non-human subjects research determination. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Access to clinical pathology archival tissue | The BTRF is the institution’s designated point of contact for requesting materials from the Pathology Archives for research. As a byproduct of the standard histologic processing that occurs during the clinical activities of the UVA Health System, tissue samples are present as FFPE blocks in the archives of the Department of Pathology. These samples represent biopsies, surgical resections and autopsies. This requires appropriate UVA Institutional Review Board for Health Sciences Research (IRB-HSR) approval or non-human subjects research determination. Material available from archival cases is generally limited to recut histologic sections or core punches. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Clinical trial and clinical research support | The BTRF supports clinical trial and clinical research by providing access to archival pathology specimens, processing and aliquoting of blood specimens, isolation of mononuclear cells from peripheral blood, processing and aliquoting of urine, saliva, ascites, and other biofluid specimens. We also assist in tissue collection and processing of on-study biopsy specimens,tissue collection from surgical pathology and outpatient clinics,standard and specialized tissue sample processing and shipping logistics. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Basic standard histology services | The BTRF provides basic histology services including tissue processing, embedding ,FFPE & frozen tissues sectioning,H&E staining, cytospin preaparation,and antigen retrieval of tissue on slide. We also provide service for histology-guided macrodissection to enrich for specific cell types. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Immunohistochemistry (IHC) | The BTRF performs immunohistochemistry (IHC) on FFPE & frozen tissues, tissue microarrays (TMAs) and on cytology preparations for human and mouse tissues. BTRF offers high-quality and efficient services for IHC full assay development, protocol validation, single/multiplex staining, image collection, and data analysis/ interpretation. The BTRF maintains a core panel of IHC stains which the antibodies are provided by the BTRF. For IHC stains not on the core list, the investigator must provide the antibody(ies) and the BTRF provides all secondary and detection reagents. A “work up” charge is applied for the titration of all new antibodies. Currently the BTRF can provide multiplexing IHC up to 6 colors. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Immunofluorescence staining (IF) | The BTRF performs Immunofluorescence staining (IF) on FFPE & frozen tissues, tissue microarrays (TMAs) and on cytology preparations for human and mouse tissues. BTRF offers high-quality and efficient services for IF full assay development, protocol validation, single/multiplex staining, image collection, and data analysis/interpretation. Currently the BTRF provides multiplexing IF up to 5 fluorophores ( Dapi, FITC, mCherry/Texas red,CY5 and CY7). | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Chromogenic RNA In Situ Hybridization (CISH) | The BTRF performs chromogenic RNA in situ hybridization (CISH) on histologic tissue sections. Probe sets can be designed to any RNA of choice. Signal can be detected using standard bright field microscopy. This assay can be performed on FFPE or frozen histologic sections. At present, we offer RNA ISH assays utilizing Advanced Cell Diagnostics RNAscope™ products. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| GeoMx® DSP services | GeoMx® Digital Spatial Profiler (DSP) from NanoString is enables high-plex spatial and molecular profiling technologies by generating a whole tissue image and digital profiling data up to 10’s-1,000’s for RNA or Protein analytes in tissue specimens on slides. Whole tissue sections or TMA from FFPE or frozen samples can be used for this assay. We provide services in a full scale starting from experimental design, conducting the work, performing QC and result analysis. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Whole slide scanning and analysis | Digital pathology services include high-resolution whole slide scanning coupled with our Pathcore Flow image management system for viewing, annotation, and sharing for both brightfield and fluorecence images via Hamamatsu NonoZommer S360 and S60 scanners. BTRF uses Visiopharm ONCOTOPIX software suite for automated image analysis of both bright field and fluorescence slide images. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| DNA and RNA extraction | BTRF provides nucleic acid extraction services for both DNA and RNA. Depending on the type of the sample, we utilize the extraction method that provides the best quality and yields of nucleic acids. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Tissue Microarray (TMA) manufacture | Tissue microarray technology places up to a thousand discs of tissue on a single glass slide, which can then be used for staining and other type of assays. The BTRF can create custom TMAs for investigators, using FFPE tissue either provided by the investigator, or obtained from the archives of the UVA Pathology Department. The BTRF has also provided a ready made TMA set that surveys 159 common carcinomas, we also work with NCI-sponsored consortia groups to create other TMAs that may be obtained by UVA investigators. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Storage | BTRF offers secure biospecimen storages for liquid nitrogen,-20°C and –80°C freezer storages. All units are monitored 24/7 by SOM system control. They are connected to SOM emergency power outlet. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Instrument usage | Instrument usages include cryostat usage for frozen sectioning, Nonodrop imstrument usage for measuring amount of RNA and DNA and photomicroscope for taking pictures. We provide training for all instrument usages. Reservation can be made through iLab. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Consultation | BTRF director is available for consultion in many aspects including specimen availability, core services and experimental plans. Pathology services and consultation are also availble. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Long Term Storage | Within our secure biospecimen storage facilities, the BTRF offers a range of temperature storage options including liquid nitrogen, –80°C, and –20°C for the safe and secure storage of your biological samples. | Biorepository and Tissue Research Facility Director: Pat Pramoonjago, PhD pp6f@virginia.edu |
| Manufacturing Space Rental | Rental of certified ISO 7 cleanroom suite for cGMP manufacturing activities. Basic equipment includes: refrigerator, -20C freezer, BSC, refrigerated centrifuge, and student microscope. Some other specialized equipment available upon request. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Consultation: FDA Regulation and Clinical Trials | Consultation/guidance related to IND submissions, clinical trial design, FDA & cGMP compliance | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Consultation: cGMP Manufacturing | Consultation/guidance regarding cGMP manufacturing process and SOP development | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Manufacturing: Monoclonal and Recombinant Bispecific Antibodies | Production, isolation, purification of Monoclonal and Recombinant Antibodies. Heteroconjugation/manufacture of Bispecific Antibodies | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Manufacturing: Cell-based therapies | Cell purification, isolation, culture, transfection, transduction, and cryopreservation for cell-based therapies. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Assistance | cGMP staff general assistance and manufacturing support is available for clients of the cGMP Core upon request. Common requests include ordering, receipt, and sterilization of supplies, waste disposal, equipment setup, and more. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Education Services | The Facility Scientific Director participates in Cancer Center’s seminars to spread the awareness about the cGMP facility at UVA Campus, and delivering webinars hosted by biologic product related companies. Access to cGMP and FDA compliance education resources. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Training Services: Certification to use cGMP facility | CHT cGMP staff provide basic cGMP training to new users. During this training core personel will provide and review standard operating procedures for proper use of UVA cGMP facility. Consistent compliance with this provided procedures is critical to preventing contamination of manufactured products and will also help keep the laboratory sanitary, orderly, and in compliance with environmental monitoring and regulatory specifications. Individuals must review and understand all the procedures before getting access to cGMP. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Training Services: Gowning Qualification | Access to the GMP facility is restricted to authorized and qualified individuals. These individuals must meet the gowning requirements of initially passing 3 consecutive gowning qualifications in a one month period. Until these requirements are met, individuals are considered unqualified and may only access the GMP facility if escorted by qualified staff. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Dynamic Environmental Monitoring Services: | In-Process environmental monitoring of cGMP manufacturing process. Includes total particle counts, settling plates for airborne viable particles and RODAC touch plates for surface viable particles. Identification of viable colony forming units is available upon request. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Aseptic Processing Technique Monitoring | In-Process testing of processing technician's hands and BSC at end of aseptic processing work. Includes RODAC touch plates for BSC surface and technician's hands, total particle count of BSC environment, and settling plate for airborn viable particles in BSC. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Aseptic Processing Qualification Testing | Testing of aseptic processing technique available for clients who wish to have documented qualification testing on record. Can be designed to mimic client's manufacturing processing steps. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Quality Control (QC) Services | In-house sterility testing and endotoxin testing services. Access to off-site mycoplasma and viral clearance testing. Rental of on-site QC laboratory is available for clients who wish to conduct their own QC work. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Cryostorage | Secure, managed, short-term or long-term storage of samples or cGMP finished products in liquid nitrogen vapor or -80 freezer | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Specimen Processing | Correlative sample processing. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Specimen Shipment - Ambient or Refrigerated | IATA compliant shipment of ambient or refrigerated specimens including blood, urine, and tissue. Ambient shipments may use gel-packs. Refrigerated shipments may use ice-packs or Nano-Cool refrigeration units. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Specimen Shipment - Frozen/Dry Ice | IATA compliant shipment of frozen specimens including cells, urine, serum, and plasma. Frozen shipments are shipped on dry ice. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Specimen Shipment - Frozen/Cryoshipper | IATA compliant shipment of cryopreserved specimens in a dry nitrogen vapor shipper. | Center for Human Therapeutics cGMP Facility Director: Archana Thakur, PhD at2fx@uvahealth.org |
| Cardiopulmonary Exercise Testing/VO2peak | The gold standard for measuring cardiovascular fitness and aerobic endurance. Typically used in research studies before and after an exercise training intervention, new medication, new device therapy, or to track disease progression. Includes EKG, blood pressure, and metabolic measures. Can include echocardiography, oxygen saturations, or lactate measures. Treadmill, upright bike, or recumbent bike. | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Lactate measures | Blood lactate measurements during exercise testing or training to fine tune intensity | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Functional assessments | Paced or maximal hallway walking tests, with or without heart rate, oxygen saturations, blood pressures, distance measurements, and symptom tracking. Other functional activities by investigator request | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Supervised exercise training | Supervised exercise training on bike or treadmill with or without EKG, blood pressure, or metabolic monitoring; home exercise oversight | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Supervised exercise with EKG monitoring | Rhythm and rate monitoring via EKG before, during, and after an exercise session | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Supervised exercise with metabolic measures | Exercise session with subject wearing a metabolic mask; often used to calorie-matched exercise sessions | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Home exercise oversight | Virtual or phone follow up and guidance for subjects exercising at prescribed levels at home | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Stress echo support | Stress echo bike, with or without EKG and metabolic measures, for echocardiography during exercise. | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Ankle brachial index | Assessment of lower body circulation using blood pressures measurements on the arms and legs to compute a ratio; can be performed at rest and after an exercise stress test or any activity that causes the patient to develop claudication symptoms. | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Autonomic Testing | Assessment of heart variability using simple tests of breathing with EKG, HR, and blood pressure monitoring. | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Resting Metabolic Measures | Measurement of metabolism in a supine, fasted state to determine caloric requirements and substrate utilization. | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Determination of Body Composition Utilizing BOD POD GS | Percentage body fat, Fat mass, Fat-free mass. Can be used to track changes before and after an intervention. | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Anthropometry Measurements | Circumferences (waist, abdomen, and hip) | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Physical Activity Questionnaires | Physical activity questionnaires can be used to estimate total caloric expenditure and intensity of activity over a given period of time. | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Project Assistance and Consultation | Assistance with grant submission and protocol development. This service can also be used for subject recruitment and follow up, home exercise/activity over-sight, and data entry. | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Additional Lab Time/Space | Lab/clinical space for study needs, such as screening, examination, consenting, survey completion. Possible to reserve with staff during regular hours, or without staff before/after hours. | Exercise Physiology Core Laboratory Director: Lisa Farr, M.Ed lms5a@virginia.edu |
| Luminex Human | Multiplex bead based cytokine assays utilizing Luminex technology. We offer premade kits for mouse, human, cell signaliing, and multi-species measuring up to 48 analytes per sample. Custom kits available upon request. | Flow Cytometry Core Facility Director: Mike Solga, MS mds4z@virginia.edu |
| Luminex Mouse | Multiplex bead based cytokine assays utilizing Luminex technology. We offer premade kits for mouse, human, cell signaliing, and multi-species measuring up to 48 analytes per sample. Custom kits available upon request. | Flow Cytometry Core Facility Director: Mike Solga, MS mds4z@virginia.edu |
| Antibody Conjugation Services | Fluorescent and Metal conjugation of unlabeled antibodies for flow cytometry, image cytometry, mass cytometry, and imaging mass cytometry. | Flow Cytometry Core Facility Director: Mike Solga, MS mds4z@virginia.edu |
| 10x Genomics Single Cell Library Preps | Our single cell services will enable you to analyze transcriptomes, chromatin structures, cell surface expression and immune profiling on a cell-by-cell basis through the use of microfluidic partitioning to capture single cells and prepare barcoded, next-generation sequencing (NGS) cDNA libraries. | Genome Analysis and Technology Core Director: Katia Sol-Church, PhD ks5uq@virginia.edu |
| Next Generation Sequencing (NGS) | Our Illumina short read sequencing include one Miseq and 2 Nextseq2000. These are ideal for rapidly sequence whole genomes, deeply sequence target regions, discover novel RNA variants and splice sites, quantify mRNAs for gene expression analysis, map the epigenome, and explore the exquisite leve of complexity of your sample at the single cell level. | Genome Analysis and Technology Core Director: Katia Sol-Church, PhD ks5uq@virginia.edu |
| NGS Library Preparation | In addition to our single cell library prep we offer a series of bulk RNA seq and DNA seq services. Bring in your quantified and QC'd nucleic acids and we will generate ready to sequence indexed library prep for you many projects including metagenomics, gene profilling, Whole transcriptome, Whole small genome, CRISPR screen, and Ampli-seq. | Genome Analysis and Technology Core Director: Katia Sol-Church, PhD ks5uq@virginia.edu |
| Digital PCR | Digital PCR is a technique where the sample is partitioned into many individual reactions so that either zero, one or more target molecules are present in each reaction. It is routinely used for sensitive applications, such as copy number variation analysis, gene expression quantification and rare target detection. | Genome Analysis and Technology Core Director: Katia Sol-Church, PhD ks5uq@virginia.edu |
| Nucleic Acid Quantitation And Quality Control | We have two optical technologies in the core used to quantify nucleic acids: the Nanodrop (UV-Vis measurement) an the Qubit (fluorescence measurement). A third platform, the Tape Station, is used for qualitative analysis of DNA fragments (sizing) and QC of purified RNAs (RIN scores). | Genome Analysis and Technology Core Director: Katia Sol-Church, PhD ks5uq@virginia.edu |
| Real Time PCR | Our real time PCR Quant studio system is routinely used with SybrGreen or Taqman probes to validate candidate gene expression level (relative or absolute). As well the 96-well platform is used fr SNP genotyong using allelic discrimination probes. | Genome Analysis and Technology Core Director: Katia Sol-Church, PhD ks5uq@virginia.edu |
| 10 X Genomics Chromium X | This is the platform used to prepare emulsions that are used to generate single cell or nuclei partitions enabling the generation of single cell or single nuclei libraries for RNA seq, multiOme analysis, Immune profiling ad more. | Genome Analysis and Technology Core Director: Katia Sol-Church, PhD ks5uq@virginia.edu |
| Project development services | These are free of charge consultations that will enable you and the core to optimize your experimental design, sample processing pipeline, data troubleshooting. We also provide support for grant aplications e.g. budget prepraration, letter of support, aims reviews and for manuscript we provide write up and proofreading methods section services to ensure reporting accuracy. | Genome Analysis and Technology Core Director: Katia Sol-Church, PhD ks5uq@virginia.edu |
| CRISPR | Delivery of CRISPR/Cas9 with or without repair templates for targeted changes to the mouse genome. Done via electroporation or microinjection. Used for creation of indels, deletions, point mutations, protein tags, insertion of loxP sites, fusions, creations of bi-cistronic transcripts, or insertion at Rosa26 or other safe harbor loci. | Genetically Engineered Murine Model Core Director: Daniel Grigsby, PhD qsj6gh@virginia.edu |
| Transgene Injection | Delivery of linearized plasmid DNA to fertilized embryos by microinjection for random integration into the genome | Genetically Engineered Murine Model Core Director: Daniel Grigsby, PhD qsj6gh@virginia.edu |
| Rederivation | Revival of mouse lines from sperm or embryos. From sperm, this is accomplished by in vitro fertilization (IVF) and generally uses frozen sperm. Materials can come from in-house stored material or materials shipped from external sources. | Genetically Engineered Murine Model Core Director: Daniel Grigsby, PhD qsj6gh@virginia.edu |
| Workshop | A three day workshop that covers the various methods to create genetically engineered mice, with a specific focus on CRISPR technology. Topics include project design, screening and sequencing of founder mice, and demonstrations of microinjection and electroporation in the lab. | Genetically Engineered Murine Model Core Director: Daniel Grigsby, PhD qsj6gh@virginia.edu |
| Cryopreservation | Freezing of either sperm or embryos to preserve a mouse line. Samples are stored in-house in liquid nitrogen. Shipments to other institutions or investigators can be arranged by the PI | Genetically Engineered Murine Model Core Director: Daniel Grigsby, PhD qsj6gh@virginia.edu |
| Electron Cryo-Microscopy (Cryo-EM) | Single particles from solutions with potential resolution < 1nm Helical and two-dimensional crystals Some atomic models rival X-ray crystallography and NMR | Molecular Electron Microscopy Core Director: Michael Purdy, PhD mpurdy@virginia.edu |
| Electron Cryo-Tomography (Cryo-ET) | 3-D maps of non-regular structures, larger cellular complexes and cell sections resolution 3-4 nm for irregular structures, higher for regular structures | Molecular Electron Microscopy Core Director: Michael Purdy, PhD mpurdy@virginia.edu |
| Micro Electron Diffraction (MicroED) | Electron diffraction of micro-crystals, resolution can reach 1-2Å | Molecular Electron Microscopy Core Director: Michael Purdy, PhD mpurdy@virginia.edu |
| Computational Services | Direct support in setting up appropriate servers or workstations, limited data analysis for single-particle and tomographic projects, guidance and suggestions for data processing | Molecular Electron Microscopy Core Director: Michael Purdy, PhD mpurdy@virginia.edu |
| TEM Sample Preparation | Fixing, dehydrating, and embedding biological samples before ultrathin sectioning and staining to enhance contrast | Molecular Electron Microscopy Core Director: Michael Purdy, PhD mpurdy@virginia.edu |
| TEM imaging | Transmission Electron Microscopy imaging uses a focused beam of electrons that passes through an ultra-thin sample to produce highly detailed, high-resolution images of the sample's internal structure. | Molecular Electron Microscopy Core Director: Michael Purdy, PhD mpurdy@virginia.edu |
| Cryo-EM Grid Preparation | Rapidly freezing biological samples or soft materials in a thin layer of vitreous ice to preserve their native structure for electron microscopy | Molecular Electron Microscopy Core Director: Michael Purdy, PhD mpurdy@virginia.edu |
| Negative Stain Grid Preparation | Enhance contrast by surrounding the sample with an electron-dense stain. Creates a high-contrast outline of the sample, making it easier to visualize structures like proteins and viruses | Molecular Electron Microscopy Core Director: Michael Purdy, PhD mpurdy@virginia.edu |
| Single Particle Cryo-EM Structure Determination | Technique used to analyze the 3D structure of macromolecules at near-atomic resolution. Widely used for studying the structure of proteins, complexes, and viruses in their near-native environments | Molecular Electron Microscopy Core Director: Michael Purdy, PhD mpurdy@virginia.edu |
| Cryo-ET Data Processing | Reconstruct a high-resolution 3D structure of biological specimens from a tilt series of 2D images. Cryo-ET is particularly useful for studying the 3D organization of cellular structures in their native environment. | Molecular Electron Microscopy Core Director: Michael Purdy, PhD mpurdy@virginia.edu |
| Imaging Services | MRI, PET, SPECTX-Ray CT, Fluorescence, Luminescence | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| MRI rodent brain morphometry | Magnetic Resonance Imaging (MRI) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| MRI in vivo tumor size measurement (longitudinal) | Magnetic Resonance Imaging (MRI) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| MRI cardiac function, T1 mapping, extracellular volume fraction (fibrosis), perfusion | Magnetic Resonance Imaging (MRI) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| MRI body composition and/or fat fraction of organs (e.g. liver, kidney) | Magnetic Resonance Imaging (MRI) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| MRI guided ultrasound: openeing the Blood Brain Barrier for targeted drug delivery | Magnetic Resonance Imaging (MRI) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| MRI guided ultrasound: transcranial neuromodulation | Magnetic Resonance Imaging (MRI) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| Advanced in vivo brain tissue characterization and function (DTI, MRA, SWI, perfusion, fMRI) | Magnetic Resonance Imaging (MRI) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| High resolution ex vivo structural MRI (T1w, T2w, DTI) | Magnetic Resonance Imaging (MRI) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| Wide variety of PET tracers available to study disease processes in vivo | Positron Emission Tomography (PET) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| Study glucose metabolism using 18F-FDG | Positron Emission Tomography (PET) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| Tumor detection using various SPECT tracers | Single Photon Emission Computed Tomography (SPECT) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| Very high resolution in vivo structural imaging | Micro-computed tomography (microCT) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| Very high resolution ex vivo imaging of fixed tissue, bone density, organ vasculature | Micro-computed tomography (microCT) | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| Monitoring tumor growth and effec of drugs with luciferase | Optical imaging | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| In vivo monitoring of biological processes using fluorescent tracers | Optical imaging | Molecular Imaging Core Director: Maurits Jansen, PhD vtf5vq@virginia.edu |
| [18F]Sodium Fluoride | Bone tumors | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]DPA-714 and [18F]FEPPA | Translocator protein (TSPO) ligand for neuroinflammation or microglia activation | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]FBCTT | Dopamine transporter ligand | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]Fallypride | Dopamine D2/D3 receptor radioligand | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]FB-IL2 | A marker for CD25+ T-lymphocytes | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]FDOPA | Neuroendocrine tumors | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]FET | Primary brain tumors | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]DCFPyl | Prostate Cancer – PSMA | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]FAZA | Hypoxic tumor microenvironment – predictor of radiation response | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]Fcholine | Metastatic prostate cancer, recurrent brain tumor, hepatocellular carcinoma | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]FLT | Cell proliferation, head-and-neck tumors | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]FMISO | Tumor hypoxia status | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]FPIA | Aberrant lipid metabolism and cancer detection | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [18F]FEOBV | Vesicular acetyl choline transporter tracer | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [11C]Carfentanil | Mu (μ) opioid receptor | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [11C]Choline | Metastatic prostate cancer, recurrent brain tumor, hepatocellular carcinoma | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| [11C]Methionine | Monitoring radiation therapy of cancer | Radiochemistry Core Director: Shivashankar Khanapur, PhD krs5xf@virginia.edu |
| Tissue processing & embedding | Dehydration, clearing and infiltration of tissue with paraffin wax. | Research Histology Core Director: Sheri Vanhoose slv4e@virginia.edu |
| Paraffin-embedded and frozen tissue sections | Cutting thin slices of tissue either paraffin or frozen then mounting on slide for microscopic evaluation | Research Histology Core Director: Sheri Vanhoose slv4e@virginia.edu |
| Special stain | Hematoxylin and Eosin, Safranin O, Oil Red O, Aldehyde Fuchsin, Giemsa, Periodic Acid Schiff (PAS), Gomori’s Trichrome, Masson’s Trichrome, Picrosirius Red, Toluidine Blue | Research Histology Core Director: Sheri Vanhoose slv4e@virginia.edu |
| Immunohistology | Research Histology Core Director: Sheri Vanhoose slv4e@virginia.edu |
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| RNA extraction (FFPE, Fresh Tissue, and Cell line) | RNA extraction for RNAsequence, Nanostring nCounter, DV200, QC quality control, qPCR. | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
| RNA clean and concentrator | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
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| Tissue Disaggregator - Cytiva VIA Extractor | Sample preparation for single-cell experiments (Isoplexis, Single-Cell NGS analysis, Flow Cytometry, Cell Culture, Mass Spectrometry | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
| BSL2 Laboratory rental | Leasing a space that meets specific biosafety standards required for handling moderate-risk biological agents | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
| iPSC cell culture | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
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| iPSC cell maintenance | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
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| Single-Cell Secretome | Monitor 32+ cytokine analysis at single-cell resolution | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
| Single-Cell Signaling | Highly multiplexed phosphoprotein analysis at single-cell resolution | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
| CodePlex: Multiplexed Bulk Cytokine | Target 30+ Cytokines: 3 panels option for mouse and human. Monitoring 8 samples per chip. | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
| Meteor: Quantitative Bulk Cytokine | Run 20 samples in triplicate on each chip; Analyze 10 cytokines (GM-CSF, IFN-g, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-17A, TNF-a) | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
| CosMx - Single-Cell Resolution In Situ (NanoString/Bruker) | Human 6K Discovery RNA PanelProbe Base Transcriptomics and Proteomics Analysis with single-cell resolution. Panels Available: Human 1K Universal Cell Characterization RNA Panel, Human Immuno-Oncology Protein Panel (64 targets), Mouse 1K Neuroscience RNA Panel, Mouse 1K Universal Cell Characterization RNA Panel, Mouse Neuroscience Protein Panel (68 targets), Custom RNA Barcoding Service | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
| Visium HD (8 um resolution) | Visium HD, Human Transcriptome, 6.5 mm, 4 rxns, Visium HD, Mouse Transcriptome, 6.5 mm, 4 rxns | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
| Visium V2 (55 um resolution) | Visium V2, Human Transcriptome, 6.5 mm, 4 rxns, Visium V2, Human Transcriptome, 11 mm, 2 rxns, Visium V2, Mouse Transcriptome, 6.5 mm, 4 rxns, Visium V2, Mouse Transcriptome, 11 mm, 4 rxns | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |
| NanoString nCounter | It is a robust method for multiplexing up to 800 gene expression targets using direct detection technology, producing highly reproducible data, and requiring no amplification or technical replicates. This platform offers different panel that can be used in numerous fields of science: Oncology, Immunology, Infectious Disease, Neuroscience, Cell & Gene Therapy, Gene Signatures, Cardiovaascular Disease | Spatial Biology Core Director: Ana Karina de Oliveira, PhD ak4yj@virginia.edu |