GFP Actin Mouse Movie
Inner ear sensory organs from transgenic mice that express a γ-actin-GFP fusion protein (Fischer et al., 1998; Fischer et al., 2000) exhibit intense fluorescence in structures that are F-actin-rich. Movie shows the 3D rendering of a confocal stack acquired from an intact, live utricle, shortly after dissection from the ear of a γ-actin-GFP mouse. The stack is centered on the apical surface of the sensory epithelium. F-actin-rich stereocilia bundles protrude from the surface of hair cells, and circumferential bands of F-actin line the apical junctions of neighboring supporting cells.