Metabolomics & Lipidomics Shared Resource
The Cancer Center Shared Resource for Metabolomics & Lipidomics at the University of Virginia is an analytical facility specializing in mass spectrometry techniques for assessing small molecules. These approaches encompass a variety of targeted, untargeted, qualitative, and quantitative analyses to support investigators in their research. The core has experience with a variety of sample types, including mammalian (cells, tissues, fluids), plants, insects, and biomaterials. Example analyses include the following:
The Core can aid in determining various in vitro and in vivo properties of drugs. Examples include, microsomal stability, half-life, protein binding, and determination of phase I and II metabolites.
Mass spectrometry has applicability beyond the above listed categories. We are open to applying new approaches and developing new methods to aid investigators in their research needs.
Various methods are available for targeted analyses of metabolites to determine steady-state levels. These include amino acids, organic acids, short-chain fatty acids, eicosanoids, acyl-CoAs, acyl-carnitines, sphingolipids, and more. Assays can be developed for your compound(s) of interest.
Flux analyses serve to test mechanisms for altered steady-state levels of metabolites. Stable isotope labeled compounds (e.g. 13C, 2H and/or 15N) are used to assess the activity through a pathway.
Generally a hypothesis-generating experiment, these approaches will profile changes in metabolites between groups.
Overview of Services
- The UVA Metabolomics & Lipidomics Shared Resource provides a diverse array of small molecule mass spectrometry-based analyses. These encompass a variety of targeted, untargeted, qualitative, and quantitative analyses.
- Examples include, but are not limited to; lipid profiling, metabolomics, drug pharmacokinetics and phase I & II drug metabolite identification.
- The core has experience with a variety of sample types, including mammalian (cells, tissues, fluids), plants, insects, and biomaterials